红细胞CD58在健康施灸人群中的表达:40例变化分析

Erythrocyte CD58 expression in healthy population undergoing moxibustion:An analysis of 40 cases

背景:CD58是一种糖蛋白,属于免疫蛋白超家族成员,广泛表达于人体各种免疫细胞和红细胞,是红细胞调控T细胞免疫功能的重要天然免疫物质基础。目的:通过在神阙、关元等传统保健穴上施灸,观察对健康人群红细胞CD58表达的影响。设计、时间及地点:前后对照、调查分析。于2008-03/12在山西中医学院实验中心完成。对象:选择山西中医学院在校健康大学生40例,男女各20例,平均年龄21.08岁。受试者均知情同意。方法:将以熟地、山药、山茱萸等为主方的药物制成药饼置于受试者神阙、关元、足三里、脾俞、肾俞等穴施灸,隔日1次,共灸10次,进行施灸前后的对比。主要观察指标:在施灸前、后分别进行血细胞的常规检查,流式细胞仪测定红细胞表面CD58分子的阳性百分率和平均荧光强度。结果:施灸后红细胞、白细胞数量较施灸前显著升高(均为P<0.01);与施灸前相比,红细胞CD58分子的阳性百分率和平均荧光强度均明显高于施灸前(均为P<0.01)。其中,红细胞CD58荧光强度的变化尤为突出。结论:红细胞CD58分子数量表达与艾灸密切相关,隔药饼灸增强了机体的免疫调节功能。

BACKGROUND：CD58 is a glycoprotein,belongs to immune protein superfamily members,widely expresses in various human immunocytes and erythrocytes,and is the key immune substance of regulating T cell immune function by erythrocytes.OBJECTIVE：To explore the influence of moxibustion on the acupoints of Shenque （CV8） and Guanyuan （CV4） on erythrocyte CD58 expression in healthy people.DESIGN,TIME AND SETTING：A randomized,controlled experiment was performed at the Central Laboratory,Shanxi College of Traditional Chinese Medicine from March to December 2008.PARTICIPANTS：A total of 40 healthy undergraduates,20 males and 20 females,aged mean 21.08 years,admitted in Shanxi College of Traditional Chinese Medicine were selected.Informed consents were obtained from each participant.METHODS：Putting the medicinal cakes on the acupoints,Shenque （CV8）,Guanyuan （CV4）,Zusanli （ST36）,Pishu （BL20）,Shenshu （BL21）,and then lighted moxa every other day,totally for 10 times.Different quantities were compared before and after applying moxibustion.MAIN OUTCOME MEASURES：Hemocyte routine examination was performed before and after moxibustion.Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were determined by flow cytometry.RESULTS：The quantities of erythrocytes and leukocytes were increased significantly following moxibustion （P〈0.01）.Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were significantly greater following moxibustion （P〈0.01）,especially fluorescent strength of CD58 molecule on erythrocyte surface following moxibustion.CONCLUSION：There are close relationships between CD58 expression and moxibustion.Moxibustion with medicinal cake plays an important role in the immune function of a body.