2004-2008年我国流行的A型流感病毒抗原性及HA1基因特性的研究

Study on the antigenicity and HA1 gene characteristics of influenza A viruses during 2004-2008 year in China

目的了解我国2004-2008年A（H1N1、H3N2）型流感病毒流行情况、抗原性和基因特性变异关系，了解疫苗株与我国流行株之间抗原性变化情况。方法选择2004年以来我国分离的A（H1N1、H3N2）型流感病毒进行抗原性及HA1区基因序列，通过比对HA1蛋白位点变异情况，分析我国流感病毒抗原性及基因特性变化情况。结果A（H1N1）亚型流感毒株抗原性2004-2007年分离的A（H1N1）亚型流感病毒的抗原性与疫苗株A／New Caledonia／20／1999（H1N1）类似；2008年我国流行的A（H1N1）亚型毒株的抗原性与2008-2009年北半球的流感疫苗株A／Brisben／59／2007（H1N1）类似。2004-2005年分离的A（H3N2）亚型流感病毒的抗原性与疫苗株A／Fujian／411／12002（H3N2）比较发生了变异；2006-2007年我国流行的H3N2毒株与A／Wiscansin／67／2006（H3N2）类似，2008年我国流行的H3N2毒株与疫苗株A／Brisben／10／2006（H3N2）类似。结论2004-2008年我国流行的A（H1N1、H3N2）亚型流感病毒的抗原性和基因特性发生了改变。

Objective To under stand influenza A viruses epidemic, antigenicity and genetic characteristics variation between the vaccine and Circulation strains during 2004-2008 year in China. Methods The influenza A viruses（H1N1, H3N2） isolated from 2004-2008 year were under took antigenic and sequence analysis. Influenza A virus antigenicity and genetic characteristics were analyzed thought amino acid variation compassion of HA1 protein of influenza A virus isolates. Results The antigenicity of influenza H1N1 subtype viruses isolated from 2004 to 2007 is very similar with vaccine strain A/New Caledonia/20/1999（H1N1）-like virus. The influenza H1N1 viruses circulated in 2008 year had similar antigenic characteristics with A/Brisben/59/2007 （H1N1） which is component of influenza vaccines for northern hemisphere 2008-2009 year. The influenza H3N2 subtype viruses of 2004-2005 year had antigenic variation comparatively with vaccine strain A/Fujian/411/12002 （H3N2）, The antigenicity of 2006-2007 H3N2 viruses and 2008 year＇s is A/Wiscansin/67/2006（H3N2） and A/ Brisben/10/2006（H3N2） respectiyely. Concluion There is change of influenza A viruses（H1N1, H3N2） antigenic and genetic characteristics during 2004-2008 in China.