摘要
目的通过HPV16 E5序列进化分析,初步研究中日友好医院妇产科宫颈病变诊治中心无宫颈病变的汉族妇女HPV16型内变异株类型。方法用PCR法扩增HPV16 E5基因片段,并进行比对分析测序。比对后的结果结合临床资料进行分析。结果本研究第一次利用只有236bp碱基的E5序列进行HPV16变异株的进化分析,发现单独使用E5序列进行HPV16变异株进化分析的准确率很高,并且,本研究第一次发现以4075T即可将亚洲株(As)变异株和其他变异株区分开来。结论从E5基因序列出发进行进化分析,准确率高。单独以4075T即可将As变异株和其他变异株区分开来。
Objective To investigate the distribution of HPV 16 variants in Han women patients without Cervical intracpithelial neoplasia in the diagnosis and treatment center for cervical disease, department of Obstetrics and Gynecology in China-Japan friendship hospital with HPV 16 E5 sequence phylogenetic analysis. Methods PCR amplification of HPV 16 E5 sequences and sequenced. The association between variations types and different cervical lesions was analyzed. Results In this research, We first found that variant classification based on HPV 16 E5 DNA sequence (236 bp) alone had high rate of accuracy. In addition, for the first time, our research revealed that single-4075T can distinguished the As variant from all other variants. Conclusion If E5 sequence was used for phylogenetic analysis, it will greatly reduce the experimental costs and improve efficiency and cost- effectiveness. For the first time, our research revealed that single 4075T can distinguished the As variant from all other variants.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第6期452-454,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家科技部支撑计划(2008DA156B01).