摘要
目的据北京友谊医院本院艾滋病初筛实验室2008年检测结果,经过计数分析,以评估艾滋病的发病趋势,为今后早发现阳性病例,从而有效控制艾滋病的传播。方法所有血清标本均用ELISA法初筛.初筛阳性标本用明胶凝集快速法(PA)复检,两种方法均为阳性或一阴一阳者送北京市疾病预防控制中心性病艾滋病防治所进行免疫蛋白印记法(Western Blot)确认。结果2008年度累计检测人数21467人,初筛结果阳性29份,确认结果阳性7份,确认可疑阳性12例,初筛阳性率13.5%0,确认阳性检出率24.1%(7/29),可疑阳性率41.4%(12/29)。并对标本来源,年龄和性别进行一定的流行病学分析。结论应用ELISA法进行艾滋病初筛检测具有实际意义,具有准确性高、判断结果较准确,便于记录结果,适合艾滋病的初筛检测。
Objective According to test results of the Hospital of AIDS screening laboratory in 2008, after counting analysis to assess the prevalence of AIDS, we can early detect positive cases in the future and effectively control the spread of AIDS. Methods All serum samples were screened by ELISA method and we reexaminated the samples by PA. As long as one result is positive by the two methods, then we sent the positive samples to Beijing Center for Disease Control and Prevention by Western Blot method to confirm the result. Results A total of 21 467 samples were detected and 29(13.5%0) were positive screening results. We confirm there were 7(24.1%) positive samples and 12(41.4%) suspected samples. We researched the epidemiology of the specimens by its source and age and sex. Conclusion Application of ELISA method for HIV screening test has a practical significance, it is accurate and fit to record the results of the screening test for AIDS.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第6期491-492,共2页
Chinese Journal of Experimental and Clinical Virology
关键词
HIV
酶联免疫吸附测定
HIV
Enzyme-Linked Immunosorbnent Assay