兔舒张性心衰和收缩性心衰模型建立及间质重构的比较

Comparison of interfitial remodeling in different rabbit models with diastolic heart failure and systolic heart failure

目的比较兔舒张性心衰(DHF)和收缩性心衰(SHF)模型间心功能和间质重构的差异。方法采用腹主动脉缩窄术建立DHF模型,用腹主动脉缩窄术联合破主动脉瓣术建立SHF模型,用心超和血流动力学检测心功能,用羟脯氨酸法测定心肌胶原含量,天狼猩红染色(PSR)测定胶原面积(CA)、容积分数(CVF)及Ⅰ/Ⅲ型胶原的面积比值。结果与对照组相比,DHF组心肌明显肥厚,僵硬度增加,心室舒张功能异常但射血分数(EF值)正常,间质胶原含量、面积、容积分数及Ⅰ/Ⅲ型面积比值均显著升高;SHF组心腔明显扩大,心室收缩功能异常,间质胶原含量、面积及容积分数明显升高,但Ⅰ/Ⅲ型面积比值下降(P<0.05或P<0.01)。结论成功构建了可以代表不同临床类型的两种心衰模型,为以后的实验研究提供了技术支持。

Objective To compare the differences of cardiac function and interstitial remodeling between diastolic heart failure（DHF） and systolic heart failure（SHF） rabbit models.Methods To establish DHF model with abdomial aorta constriction and SHF model with abdomial aorta constriction plus aortic insufficiency.The cardiac function was examined by UCG parameters and homodynamic parameters.The collagen content was measured through hydroxyproline colorimetric assay and shown as collagen area（CA）,collagen volume fraction（CVF） and area ratio ofⅠto Ⅲ type collagen with PSR.Results Compared with control group,there were significantly increased thickness and stiffness of myocardium,impaired diastolic function but normal ejection fraction（EF）,and significantly increased collagen content,CA,CVF and area ratio ofⅠto Ⅲ type collagen in DHF group;heart chamber was significantly enlarged,systolic function decreased,and collagen content,CA,CVF significantly increased,but ratio ofⅠto Ⅲ type collagen decreased in SHF group（P〈0.05 or P〈0.01）.Conclusion DHF and SHF rabbit models were established successfully,which can simulate clinical profiles and provide technical support to future research.