葛根素减轻乙醇导致大鼠生精细胞的凋亡

Puerarin reduces ethanol-induced-apoptosis of spermatogenic cells in rat testis

目的观察乙醇导致大鼠生精细胞的凋亡及葛根素的干预。方法将大鼠30只随机均分为对照组、乙醇组及葛根素干预组。于实验第40天免疫组织化学法(SABC)检测左侧睾丸各组BCL-2、BAX蛋白在生精细胞的表达;RT-PCR检测右侧睾丸各组BCL-2及BAXmRNA的表达;TUNEL法检测生精细胞的凋亡。结果乙醇组平均每个生精小管断面中BCL-2蛋白阳性细胞数和A值低于对照组(P<0.01),而平均每个生精小管断面中BAX蛋白的阳性细胞数和A值高于对照组(P<0.01);乙醇组BAXmRNA表达较葛根素干预组及对照组强(P<0.05),而BCL-2mRNA表达较葛根素干预组及对照组弱(P<0.05);乙醇组每个生精小管横切面中的凋亡细胞数目高于对照组(P<0.01),葛根素干预组显著缓解上述变化。结论葛根素对乙醇导致的大鼠生精细胞凋亡有干预作用。

Objective To investigate potential protection by protect against ethanol-induced apoptosis of spermatogenic cells in rat testis.Methods Thirty SD adult male rats were randomly divided into three groups： normal group,alcohol group and puerarin group.At 40th day,BCL-2 and BAX of spermatogenic cells of testis tissue were checked by RT-PCR and immunohistochemistry;Apoptosis of spermatogenic cells was determined by TUNEL.Results The results of RT-PCR and immunohistochemistry indicated that BCL-2 and BAX of spermatogenic cells were not significanty different between puerarin group and normal group,but there was the significant difference between alcohol group and puerarin group（P〈0.01）.Apoptosis of spermatogenic cells in alcohol group was significantly higher than normal group.Conclusion Spermatogenic cells could generate apoptosis by changing the expression of BCL-2 and BAX.Puerarin could inhibit this damage of didymus by alcohol.