AHSG基因多态性与老年女性动脉硬化及骨质疏松的关系

Relationship of polymorphism of alpha2-HS glycoprotein gene with atherosclerosis and osteoporosis in elderly female

目的探讨老年女性α2-HS糖蛋白（AHSG）基因多态性与动脉硬化以及血清骨相关生化指标的关系。方法用ELISA法测定344名住院2080岁女性患者骨特异性碱性磷酸酶、Ⅰ型胶原羧基末端肽、Ⅰ型胶原氨基末端肽和护骨素的浓度;对患者基因组的DNA样品作限制性内切酶SacⅠ的PCR-RFLP检测,以确定其基因型,全自动生化仪酶法测定血清TC、TG、HDL-C、LDL-C,电极法测定血钙,DXA测定腰椎正位、仰卧侧位腰椎和股骨颈的骨密度（BMD）。GG基因型患者做骨组织病理活检,彩色超声诊断仪测定颈动脉中内膜厚度（IMT）。结果（1）老年女性患者AHSG基因CC型、CG型和GG型分布频率分别为59.7%、25.1%和15.2%。不同基因型血脂、血Ca^2＋和骨生化指标有明显差异;（2）协方差分析显示,正侧位腰椎、股骨颈的BMD和IMT与老年女性CG＋GG基因型呈线性正相关趋势;（3）GG型老年女性患者骨组织病理切片和彩超验证了AHSG基因变异与动脉硬化、骨质疏松发生发展的相关性。结论AHSG基因多态性变异与老年女性动脉硬化和骨质疏松的发病密切相关。

Objective To investigate the distribution of alpha2-HS glycoprotein（AHSG） gene polymorphisms and the relationships of AHSG gene polymorphisms with atherosclerosis as well as serum bone related biochemical markers.Methods Blood samples of 344 hospitalized female patients,aged 20～80 years,were sampled for serum bone alkaline phosphatase,cross-linked N-telopeptide of collagen typeⅠ,cross-linked C-telopeptide of collagen type Ⅰ,osteoprotegrin and leptin were determined by ELISA.Serum TC,TG and calcium content were detected.Polymorphism of AHSG gene was detected by polymerase chain reaction fragment length polymorphisms（PCR-RFLP） of restriction enzyme Sac Ⅰ.BMD（Norland XR-36） of the anteroposterior spine（AP）,supine lateral spine（Lat） and femoral neck（FN） were measured.Morphological changes in the aorta and bone of type GG patient were detected by pathological microscopy.IMT were measured by color doppler ultrasound equipment（SEQUOIA512）.Results（1） The genotype frequency of CC,CG,and GG were 59.7%,25.1% and 15.2% respectively in all elderly female patients.There were significant difference in blood lipids,Ca^2＋ and serum bone relative biochemical markers to different AHSG genotypes.（2）There were significant differences in the BMD of the AP,Lat,FN and IMT and the serum biochemical markers among the CC,CG and GG genotypes.（3）GG-female patients bone tissue pathology section verify the AHSG polymorphism genetic mutation and atherosclerosis,osteoporosis development of the relationship.Conclusion There was close relationship among AHSG polymorphism variation and the incidence of arteriosclerosis and osteoporosis in elderly female.