截敏祛风汤对变应性鼻炎大鼠鼻黏膜组织的影响

Effect of Jieminqufeng decoction on acetylcholine in nasal mucosa of the rats with allergic rhinitis

目的观察截敏祛风汤对变应性鼻炎(allergic rhinitis,AR)大鼠的治疗作用,探讨其作用机制。方法选择健康大鼠40只,随机分为截敏祛风汤组(A组)、西替利嗪组(B组)、模型对照组(C组)和正常对照组(D组)。造模成功后分别给药7天,观察大鼠行为学变化,计数鼻腔分泌物中嗜酸性粒细胞(eosinophile granulocytest,EOS);观察鼻黏膜组织形态学改变,检测乙酰胆碱酯酶(acetylcholine esterase,AchE)活性。结果A、B组大鼠过敏症状较C组明显减轻,与D组相近;A、B、D组鼻分泌物中EOS个数明显低于C组;各组大鼠鼻黏膜组织形态学表现不同;A、B组鼻黏膜组织中AchE活性升高,与C组比较有显著差别(P<0.05);C组和D组AchE活性相近。结论截敏祛风汤能明显改善AR大鼠的过敏症状,机制可能是通过作用于EOS,延缓或阻断变态反应进程,提高大鼠鼻黏膜组织中AchE活性,进而降低组织中乙酰胆碱含量来实现的。

OBJECTIVE To observe the therapeutic effect and its mechanism of Jieminqufeng decoction on AR of rats. METHODS Forty Wistar rats were randomly divided into 4 groups： Jieminqufeng decoction group（group A）, cetirizine group（group B）, model control group（group C）and normal control group（group D）. After the model rats of AR were established, the animals in 4 groups were given different drugs for 7 days respectively. The behavioral changes of rats, the number of EOS in rhinorrhea, the histomorphological changes and the activity of AchE in the nasal mucosa were studied. RESULTS The allergic symptoms of group A and B were similar with group D, but were less compared with that in group C. The number of EOS and the histomorphological manifestations were different among the 4 groups. The activity of AchE in group A and B was higher than that in group C and D（P〈0.05）. CONCLUSION The Jieminqufeng decoction is an effective drug to AR. Its possible mechanism is acting on EOS, increasing the activity of AchE in nasal mucosa, consequently decreasing the level of acetylcholine.