爱普列特对大鼠前列腺凋亡相关基因及5α还原酶mRNA表达的影响

Effect of epristeride on expressions of gene related apoptesis and 5 alpha-reductase mRNA in rat prostate

目的评价爱普列特对对大鼠前列腺Bcl-2、Bax、CD34、eNOS及5α还原酶mRNA表达的影响。方法分别给予雄性大鼠爱普列特（爱普列特组）、非那雄胺（非那雄胺组）和生理盐水（正常对照组），观察大鼠Bcl-2、Bax、eNOS、微血管密度（MVD）以及2种5a还原酶mRNA的表达情况。结果爱普列特组Bc1—2、eNOS、MVD（148±42、72．3±48．5、4．6±2．2）与非那雄胺组（152±59、144．8±80．8、4．9±1．3）及正常对照组（216±52、293．9±116．0、8．0±3．0）比较，3个指标均下降（F值分别为6．6、0、26．45、9．52、均P〈0．01）；Bax在爱普列特组为（390±167）、非那雄胺组为（311±155），较对照组（185±73）上升（F=6．35，P=0．004），三组Srd5α1及Srd5α2还原酶mRNA的表达差异无统计学意义（F值分别为0．427和0．58，均P〉0．05）。结论爱普列特可以促进前列腺细胞凋亡，抑制血管生成，减少微血管密度，但可能不能影响5a还原酶mRNA的表达，且其作用与非那雄胺相似。

Objective To identify the effect of epristeride on the expressions of Bcl-2, Bax, CD34, eNOS and 5 alpha-reduetase mRNA in rat prostate. Methods Male rats were fed with epritseride, finasteride or physiological saline, respectively. The expressions of Bcl-2, Bax, CD34, eNOS and 5 alpha-reductase mRNA in the prostate tissue were detected and analyzed. Results The expressions of Bcl-2, eNOS and microvessel density （MVD） were lower in epritseride group （148± 42, 72.3±48. 5 and 4. 6±2. 2） and in finasteride group （152±59, 144.8±80.8 and 4.9±1.3） than in physiological saline group （216±52, 293.9±116.0 and 8.0±3.0, F=6.60, 26.45 and 9.52 respectively, all P〈0.01）. The expression of Bax was increased in epritseride group （390± 167） and in finasteride group （311±155） than in physiological saline group （185±73, F=6.35, P=0. 004）. There were no significant differences of the expressions of 5 alpha-reductase （type 1 and type 2） mRNA among the three groups （F=0. 427 and 1.58 respectively, both P〉0.05）. Conclusions Epristeride may induce apoptosis of prostate cell, inhibit angiogenesis and reduce MVD, but it has no effect on the expressions of 5 alpha reductase （type 1 and type 2） mRNA, being similar with finasteride.