摘要
目的探讨维A酸治疗皮肤色斑的作用机制。方法应用不同浓度的维A酸作用于B16F10黑素瘤细胞及正常人黑素细胞，观察对酪氨酸酶mRNA的表达、酪氨酸酶活性、黑素含量及黑素细胞增殖率的影响。结果维A酸的作用浓度为100μmol／L时，黑素细胞酪氨酸酶mRNA表达下调均值为30．13％；当浓度为500μmol／L以上时，可使酪氨酸酶活性降低82．79％，并随着浓度的增加作用越明显；而对黑素含量的降低则需要更高的浓度（1000μmol／L）；同时，还有促进黑素细胞增殖的作用。对照组氢醌对酪氨酸酶mRNA的表达无明显影响，对色素细胞有抑制和毒性作用。结论维A酸能够抑制黑素产生，是通过下调酪氨酸酶的表达和活性来完成。
Objective To investigate the effects and mechanism of retinoic acid on B16 marina reel anoma cells and human melanocytes in vitro. Methods B16F10 routine melanoma cells and human melanocytes were cultured in culture medium which contains different concentration of components, including retinoic acid. Using reverse transcription-polymerase chain reaction （RT-PCR） mRNA expression of the tyrosinase was detected. Tyrosinase activity, melanin content and cell proliferation rate were also deter mined. Results Retinoicacid exhibited an inhibitory effect on the expression of tyrosinase mRNA. As the concentration of retinoie acid was 100 μmol/L, treating for 72 h, the expression of tyrosinase mRNA de creased 30.13% , retinoic acid exhibited an inhibitory effect on tyrosinase activity and melanin production at high concenlration （〉500 μmol/L）, and it could promote the ceil proliferation. Retinoic acid and hy- droquinone could be cooperative at high concentration （1 000 μmol/L） , and enhanced the down regulation of tyrosinase activity and melanin content. Retinoic acid could also mitigate the inhibitory effect of hydroquinone on cell proliferation, so as to protect the cells from injury. Hydroquinone had no effect on tyrosi- nase gene expression at mRNA level. Conclusion Retinoic acid inhibits the synthesis of melanin by the genetic regulation at mRNA level.
出处
《中华医学美学美容杂志》
2009年第6期405-409,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
基金项目：天津市自然科学基金资助项目（编号：003610211）
