期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

骨髓干细胞来源的星形胶质细胞参与脑损伤后胶质界膜的形成

Bone marrow stem cell-derived astrocytes are involved in glia limitans formation in rats after brain injury
下载PDF
导出
摘要 目的探讨骨髓干细胞来源的星形胶质细胞是否参与脑损伤后胶质界膜的形成。方法将制作好的雌性Sprague-Dawley大鼠脑损伤模型24h后经尾静脉植入GFP标记的雄性Sprague-Dawley大鼠的骨髓源干细胞。在植入后2、4和8周取脑,用GFAP荧光免疫组织化学方法显示星形胶质细胞,GFP显示骨髓来源细胞,观察损伤组织边缘的胶质界膜中是否存在GFP/GFAP双阳性细胞。结果在损伤组织边缘可见骨髓干细胞来源的星形胶质细胞。结论骨髓干细胞来源星形胶质细胞参与脑损伤后胶质界膜的形成。这对脑外伤的恢复和组织工程材料的植入有重要的意义。 Objective To investigate the involvement of bone marrow stem cell-derived astrocytes （BMDSCs） in the formation of glia limitans after brain injury. Methods In a female SD rat model of brain injury, green fluorescence protein （GFP）-labeled BMDSCs from male SD rats were transplanted via the caudal vein 24 h after the injury. The rats were sacrificed at 2, 4 and 8 weeks after the transplantation, and immunohistochemistry for glial fibrillary acidic protein （GFAP） was performed to observe the astrocytes. The fluorescence emitted by GFP was observed to identify the presence of the bone marrow-derived stem cells, and the GFAP＋/GFP＋ cells in the glia limitnas were detected under fluorescence microscopy. Result The GFAP＋/GFP＋ cells were found in the glia limitans between the brain lesion and normal brain tissue. Conclusion Bone marrow stem cell-derived astrocytes is involved in glia limitans formation after brain injury, which can be of significance in brain injury recovery and implantation of engineered materials.
作者 邹西峰 朱军 李建民 张海燕 赵春礼 徐群渊
机构地区 华北煤炭医学院附属医院神经外科 首都医科大学北京神经科学研究所/北京市神经再生修复研究重点实验室/教育部神经变性疾病学重点实验室 首都医科大学细胞生物学系
出处 《南方医科大学学报》 CAS CSCD 北大核心 2009年第12期2391-2393,共3页 Journal of Southern Medical University
基金 国家自然科学基金(30430280) 中国博士后科学基金 河北省科学研究规划项目(062761899) 华北煤炭医学院科学研究基金(BS040023)
关键词 骨髓源干细胞 星形胶质细胞 胶质界膜 脑损伤 绿色荧光蛋白 bone marrow-derived stem cells astrocytes glia limitans brain injury green fluorescence protein
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献11

  • 1Bjorklund A, Hokfelt T. Classical trasmitters in the CNS: Part 1 [M]//Handbook of chemical neuroanatomy. Amaterdam: Elsevier, 1984.
  • 2Reter PJ. Gliosis following CNS jnjury: The anatomy of astrocytic scars and their influences on axonal elongation [M]//Fedoroff S, Vemadakis A. Astrocytes. New York: Academic Press, 1986: 263-324.
  • 3Lindsey RM. Reactive gliosis [M]//Fedoroff S, Vemadakis A.Astrocytes. New York: Academic Press, 1986:231-62.
  • 4Cogle CR, Yachnis AT, Laywell ED, et al. Bone marrow transdifferentiation in brain after transplantation: a retrospective study[J]. Lancet, 2004, 363(9419): 1432-7.
  • 5邹西峰,张海燕,赵春礼,李铁林,徐群渊.大鼠骨髓源干细胞在损伤脑组织中的分化趋势[J].第一军医大学学报,2004,24(10):1102-1106. 被引量：3
  • 6姜传涛,杨秋慧,刘玉军,赵焕英,杨慧,徐群渊.成年大鼠及猴骨髓基质细胞的体外培养及向神经元的分化诱导[J].神经解剖学杂志,2003,19(1):56-60. 被引量：7
  • 7鲁玲玲,苏玉金,赵春礼,邹西峰,侯少平,徐群渊,杨慧.骨髓基质细胞的分离、鉴定以及TH基因的转染与表达[J].生物化学与生物物理学报,2003,35(6):536-541. 被引量：12
  • 8Maxwell WL, Follows R, Ashhurst DE, et al. The response of the cerebral hemisphere of the rat to injury. The mature rat [J]. Philos Trans R Soc Lond B Biol Sci, 1990, 328(1250): 479-500.
  • 9张培林 主编.神经解剖学[M].北京：人民卫生出版社,1998.388-390.
  • 10Mathewson A J, Berry M. Observations on the astrocyte response to a cerebral stab wound in adult rats [J]. Brain Res, 1985, 327(1-2): 61-9.

二级参考文献32

  • 1Eglitis MA, Mezey E. Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice [J ]. Proc Natl Acad Sci USA, 1997, 94(8): 4080-5.
  • 2Priller J, Flugel A, Wehner T, et al. Targeting gene-modified hematopoietic cells to the central nervous system: use of green fluorescent protein uncovers microglial engraftment [ J ]. Nat Med, 2001, 7(12): 1356-61.
  • 3Priller J, Persons DA, Klett FF, et al. Neogenesis ofcerebellar Purkinje neurons from gene-marked bone marrow cells in vivo [J ]. J Cell Biol, 2001, 155(5): 733-8.
  • 4Brazelton TR, Rossi FM, Keshet GI, et al. From marrow to brain:expression of neuronal phenotypes in adult mice [ J ]. Science, 2000,290(5497): 1775-9.
  • 5Mezey E, Chandross KJ, Harta G, et al. Turning blood into brain:cells bearing neuronal antigens generated in vivo from bone marrow[J]. Science, 2000, 290(5497): 1779-82.
  • 6Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression [ J ]. Biotechniques. 1989, 7(9): 980-2,984-6, 989-90.
  • 7Mann R, Mulligan RC, Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus [ J ]. Cell, 1983, 33( 1): 153-9.
  • 8Maxwell WL, Follows R, Ashhurst DE, et al. The response of the cerebral hemisphere of the rat to injury. Ⅰ . The mature rat [J].Philos Trans R Soc Lond B Biol Sci, 1990, 328(1250): 479-500.
  • 9Friedenstein AJ, Deriglasova UF, Kulagina NN, Panasuk AF, Rudakowa SF, Luria EA, Ruadkow IA et al. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol, 1974, 2(2): 83 -92.
  • 10Waller EK, Huang S, Terstappen L. Changes in the growth properties of CD34^+, CD38-bone marrow progenitors during human fetal development. Blood, 1995, 86:710 -718.

共引文献29

南方医科大学学报
2009年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部