期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Pten敲除对Rad51基因表达的影响及其机制 被引量：2

Effect of Pten Gene Knock-out on Expression of Rad51 Gene and Mechanism of The Effect
原文传递
导出
摘要 目的研究Pten敲除对Rad51基因表达的影响及其可能的机制。方法采用半定量RT-PCR法和克隆形成试验比较Pten+/+MEFs与Pten-/-MEFs两种细胞中同源重组修复相关基因Rad51mRNA转录水平的差异,并比较两种细胞对辐射敏感性的差异和辐射后Rad51基因mRNA转录水平的差异及其可能的机制。结果与Pten+/+MEFs细胞相比,Pten-/-MEFs细胞中Rad51基因mRNA的转录水平和对辐射的敏感性显著降低;辐射后以及PI3K/AKT信号通路抑制剂LY-294002作用后,Rad51基因mRNA的转录水平明显增高。结论Pten缺失后Rad51基因表达异常,Pten可能通过PI3K/AKT信号通路来调控Rad51基因的表达。 Objective To investigate the effect of Pten gene knock-out on expression of Rad51 gene and the potential mechanism of the effect. Methods The mRNA transcription levels of Rad51 gene related to repair by homologous recombination in Pten＋/ ＋ MEFs and Pten-/ MEFs, the radiosensitivities of the two kinds of MEFs, as well as Rad51 mRNA transcription levels in the two kinds of MEFs after radiation, were compared by semi-quantitative RT-PCR and clone formation assay, and the potential mechanism was investigated. Results The Rad51 mRNA transcription level in Pten-/ MEFs was significantly lower than that in Pten＋ / ＋ MEFs. Compared with that of Pten＋ / ＋ MEFs, the radiosensitivity of Pten/ MEFs also decreased significantly. However, both the Rad51 mRNA transcription levels in Pten-/MEFs after radiation and after treatment with LY-294002, an inhibitor of P13K / AKT signal pathway, increased significantly. Conclusion Abnormal expression of Rad51 gene was observed after knock-out of Pten gene. Pten might regulate the expression of Rad51 gene through P13K / AKT signal pathway.
作者 莫琳 陈忠明 胡迎春 米粲 吴德昌 霍艳英
机构地区 重庆医科大学病理学教研室 军事医学科学院放射与辐射医学研究所
出处 《中国生物制品学杂志》 CAS CSCD 2009年第12期1179-1182,1186,共5页 Chinese Journal of Biologicals
关键词 PTEN RAD51 基因敲除 表达 Pten Rad51 Gene knock-out Expression
分类号 Q789 [生物学—分子生物学]
  • 相关文献

参考文献12

  • 1Li J, Yen C, l,iaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science, 1997, 275 (5308): 1943-1947.
  • 2Steck PA, Pershouse MA, Jasser SA, el al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet, 1997. 15 (4): 356-362.
  • 3Li DM, Sun H.TEPI, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta. Cancer Res, 1997, 57 ( 11 ): 2124-2129.
  • 4Shen WH, Balajee AS, Wang J, et al. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell, 2007, 128 (1): 157-170.
  • 5Baker SJ. PTEN enters the nuclear age. Cell, 2007, 128( 1 ): 25-28.
  • 6Freeman D, Lesche R, Kertesz N, et al. Genetic background controis tumor development in PTEN-deficient mice. Cancer Res, 2006, 66 ( 13): 6492-6496.
  • 7Couto SS, Cao M, Duarte PC, et al. Simultaneous haploinsuffieiency of Pten and Trp53 tumor suppressor genes accelerates tumorigenesis in a mouse model of prostate cancer. Differentiation, 2009, 77 (1): 103-111.
  • 8Luchman HA, Benediktsson H, Villemaire L, et at. The pace of prostatic intraepithelial neoplasia development is determined by the timing of Pten tumor suppressor gene excision. PLoS One, 2008, 3 ( 12): e3940.
  • 9Sulis ML, Parsons R. PTEN: from pathology to biology. Trends Cell Biol, 2003, 13 (9): 478-483.
  • 10金问森,金一尊.Rad51的异常表达与肿瘤治疗[J].国际肿瘤学杂志,2006,33(10):721-724. 被引量：2

二级参考文献26

  • 1Lee YJ,Sheu TJ,Keng PC.Enhancement of radiosensitivity in H1299 cancer cells by actin-associated protein cofilin.Biochem Biophys Res Commun,2005,335 (2):286-291.
  • 2Knapp DC,Mata JE,Reddy MT,et al.Resistance to chemotherapeutic drugs overcome by c-Myc inhibition in a Lewis lung carcinoma murine model.Anticancer Drugs,2003,14(1):39-47.
  • 3Connell PP,Siddiqui N,Hoffman S,et al.A hot spot for RAD51 C interactions revealed by a peptide that sensitizes cells to cisplatin.Cancer Res,2004,64(9):3002-3005.
  • 4Van Veelen LR,Essers J,van de Rakt MW,et al.Ionizing radiation-induced foci formation of mammalian Rad51 and Rad54 depends on the Rad51 paralogs,but not on Rad52.Mutat Res,2005,574 (1-2):34-49.
  • 5Thacker J.The RAD51 gene family,genetic instability and cancer.Cancer Lett,2005,219(2):125-135.
  • 6Masson JY,Stasiak AZ,Stasiak A,et al.Complex formation by the human RAD51C and XRCC3 recombination repair proteins.Proc Natl Acad Sci USA,2001,98(15):8440-8446.
  • 7Lio YC,Mazin AV,Kowalczykowski SC,et al.Complex formation by the human Rad5 1B and Rad5 1C DNA repair proteins and their activities in vitro.J Biol Chem,2003,278 (4):2469-2478.
  • 8Liu Y,Masson JY,Shah R,et al.RAD51C is required for Holliday junction processing in mammalian cells.Science,2004,303 (5655):243-246.
  • 9Zhou C,Huang P,Liu J.The carboxyl-terminal of BRCA1 is required for subnuclear assembly of RAD51 after treatment with cisplatin but not ionizing radiation in human breast and ovarian cancer cells.Biochem Biophys Res Commun,2005,336(3):952-960.
  • 10Henning W,Sturzbecher HW.Homologous recombination and cell cycle checkpoints:Rad51 in tumour progression and therapy resistance.Toxicology,2003,193(1-2):91-109.

同被引文献17

  • 1Li YS, Milner PG, Chauhan AK, et al. Cloning and expression of a developmentally regulated protein that induces mitogenic and neurite outgrowth activity [J]. Science, 1990, 250 (4988): 1690- 1694.
  • 2Souttou B, Juhl H, Hackenbruck J, et al. Relationship between serum concentrations of the growth factor pleiotrophin and pleiotrophin-positive tumors [J]. J Natl Cancer Inst, 1998, 90 (19): 1468-1473.
  • 3Sehwarz DA, Katayama CD, Hedriek SM. Sehlafen, a new family of growth regulatory genes that affect thymocyte development [J]. Immunity, 1998, 9 (5): 657-668.
  • 4Bell TA, De La Casa-Esperon E, Doheity HE, et al. The paternal gene of the DDK syndrome maps to the Schlafen gene cluster on mouse chromosome 11 [J]. Genetics, 2006, 172 ( 1 ) : 411-423.
  • 5Brady G, Boggan L, Bowie A, et al. Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1 [J]. J Biol Chem, 2005, 280 (35) : 30723-30734.
  • 6Li G, Hu Y, Huo Y, et al. PTEN deletion leads to up-regulation of a secreted growth factor pleiotrophin [J]. J Biol Chem, 2006, 281 (16): 10663-10668.
  • 7Zhao L, Neumann B, Murphy K, et al. Lack of reproducible growth inhibition by Schlafenl and Schlafen2 in vitro [J]. Blood Cells Mol Dis, 2008, 41 (2): 188-193.
  • 8Neumann B, Zhao L, Murphy K, et al. Subcellular localization of the Schlafen protein family [J]. Biochem Biophys Res Commun, 2008, 370 ( 1 ) : 62-66.
  • 9Sohn WJ, Kim D, Lee KW, et al. Novel transcriptional regulation of the Sehlafen-2 gene in maerophages in response to TLR-trig-gered stimulation [J]. Mol Immunol, 2007, 44 (13): 3273-3282.
  • 10Condamine T, Le Luduec JB, Chiffoleau E, et al. Characterization of Schlafen-3 expression in effector and regulatory T cells [J]. J Leukoc Biol, 2010, 87 (3): 451-456.

引证文献2

中国生物制品学杂志
2009年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部