摘要
目的探讨肾炎宁对人肾小球系膜细胞(HMC)细胞增殖及细胞外信号调节蛋白激酶(extracellular signal-regulated protein kinase,ERK)的影响。方法应用细胞培养技术,进行HMC培养,采取血清药理学方法,制备肾炎宁药物血清,利用脂多糖(LPS)为刺激因子,应用四唑盐(MTT)比色法和酶联免疫吸附试验(ELISA)检测细胞的增殖和磷酸化ERK1/2的表达。结果肾炎宁可显著抑制HMC增殖,抑制率在12、24和48h分别为16.0%、15.4%、18.2%,LPS组磷酸化ERK1/2的含量与空白组比较明显升高(P<0.05);中药组与空白组比较,磷酸化ERK1/2含量明显降低(P<0.05);LPS+肾炎宁组与LPS组比较,磷酸化ERK1/2表达明显降低(P<0.05)。结论肾炎宁具有抑制正常培养条件及LPS刺激条件下磷酸化ERK1/2,从而发挥对肾小球硬化的治疗作用。
Objective To investigate the effect of Shenyanning on the expression of extracellular signal-regulated protein kinase of human mesangial cells(HMCs). Methods HMCs were cultured by applying cell cultural technique. The serum pharmacological method was used in the preparation of shenyanning serum. HMC proliferation was detected by MTF. The p-ERK1/2 expression was detected by enzymelinked immunosorbentassay (ELISA) taking lipopolysaccharide (LPS) as stimulating factor. Results Shenyanning significantly inhibited HMC proliferation; and the inhibition ratios of medium dose were 16. 0% , 15.4% and 18.2% when HMC were cultured for 12,24 and 48 h. The content of p-ERK1/2 in LPS group was obviously higher than that in blank group (P 〈 0. 01 ), that in shenyanning group was obviously lower than that in blank group( P 〈 0. 05 ). The expression of p-ERK1/2 in LPS combined Shenyanning group was obviously lower than that in LPS group (P 〈 0. 01 ). Conclusions Shenyanning could inhibit the expression of p-ERK1/2 in the condition of normal or LPS stimulation, which may be the mechanism of Shenyanning in the delay of glomerularsclerosis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第24期3163-3165,共3页
Chinese Journal of Gerontology
基金
河北省科技攻关计划项目(No.00276205D)
关键词
肾炎宁
系膜细胞培养
增殖
细胞外信号调节蛋白激酶
Shenyanning
Mesangial cell culture
Proiferation
Extracellular signal-regulated protein kinase