仙茅提取物体外抗氧化活性研究

Study on antioxidant activities of extracts from Curculigo orchioides in vitro

目的探讨仙茅提取物的抗氧化活性。方法先用80%乙醇,然后用蒸馏水对仙茅进行提取,其中醇提浸膏依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取;水提部分经醇沉,制备仙茅粗多糖。分别观察仙茅各提取物对羟基自由基(.OH)、1,1-二苯基-2-苦基苯肼自由基(DPPH.)的清除率以及对Fe3+还原力。结果与抗坏血酸比较,仙茅提取物对自由基均有较强清除作用,对.OH的清除率乙酸乙酯萃取物>正丁醇萃取物>粗多糖>总醇提物,IC50值分别为0.247、0.358、0.796和0.871mg/ml;对DPPH.的清除率乙酸乙酯萃取物>正丁醇萃取物>总醇萃取物>氯仿萃取物>石油醚萃取物>粗多糖,其IC50值分别为0.026、0.055、0.620、0.071、0.122和0.275mg/ml。不同提取物对Fe3+有较强还原能力,还原力大小为:乙酸乙酯萃取物>正丁醇萃取物>总醇提物>氯仿萃取物>石油醚萃取物>粗多糖。结论仙茅具有明显的抗氧化活性,在抗衰老方面有广泛的应用前景。

Objective To study on antioxidant activities of extracts from Curculigo orchioide in vitro. Methods Cureuligo orchioides were treated with 80% ethanol and then the residue were extracted with water. The ethanol extract were extracted respectively using petroleum ether, chloroform, ethyl acetate, n-butanol. At the same time, crude polysaccbaride was obtained by ethanol precipitation from water extract. Hydroxyl free radical（ · OH）, DPPH free radical （DPPH · ） and deoxidization Fe^3 ＋ reaction system were used to observe in vitro antioxidant effects of the above extracts. Results Compared with the ascorbic acid, the extracts had strong scavenging effect on free radicals, the activities sequence for scavenging · OH were ethyl acetate extract 〉 n-butanol extract 〉 polysaccharide 〉 ethanol total extract, which corresponding IC50 values were 0. 247, 0. 358, 0. 796 and 0. 871, respectively. The capabilities of scavenging DPPH were ethyl acetate extract 〉 n-butanol extract 〉 ethanol total extract 〉 chloroform extract 〉 petroleum ether extract 〉 polysaccharide, and the corresponding IC50 values were 0. 026, 0. 055, 0. 620, 0. 071, 0. 122 and 0. 275 mg/ml, respectively. Different extracts had strong deoxidization ability of Fe^3 ＋ , the activities sequence were ethyl acetate extract 〉 n-butanol extract 〉 ethanol total extract 〉 chloroform extract 〉 petroleum ether extract 〉 polysaccharide. Conclusions Curculigo orchioides have stronger antioxidative activities and will be widely applicated on antiaging.