塑性变形纳米化结构对小鼠成骨细胞骨架肌动蛋白影响的研究

Effects of nanostructured titanium fabricated via surface plastic deformation on MC3T3 actin cytoskeleton

目的研究纯钛表面塑性变形纳米化结构对小鼠成骨细胞系MC3T3细胞骨架肌动蛋白的影响。方法采用塑性变形技术使纯钛表面纳米化,应用激光共聚焦显微镜检测钛板表面纳米化程度。应用罗丹明-鬼笔环肽染色细胞,在激光共聚焦显微镜下观察细胞接种于纳米化钛板表面和对照钛板表面不同时间点后,MC3T3细胞骨架肌动蛋白的变化情况。结果激光共聚焦显微镜下观察发现,纳米化钛板表面的结构早期能促进细胞骨架肌动蛋白纤维更充分的伸展,微丝纤维更清晰,6-12h差异最为明显,至24h后差异变得不明显。结论纯钛表面塑性变形纳米化结构有利于MC3T3细胞的黏附、铺展和细胞骨架蛋白的充分伸展。

Objective To investigate the effects of nanostructured titanium fabricated via surface plastic deformation on MC3T3 actin cytoskeleton. Methods We prepared nanostructured titanium surfaces using plastic deformation method. Surface characterization was detected by Laser Confocal Scanning Microscope（LCSM）.The nanostructured titanium was examined as experimental group,while the untreated titanium was used as control group.The effects of nanostructured titanium surfaces on MC3T3 actin cytoskeleton in different periods were examined using LCSM. Results The MC3T3 cells which grew on nanostructured titanium surface fabricated via plastic deformation had the largest spreading area and the actin filaments in cells with regular directions were well defined and fully spread. There were significant differences between two groups from 6 hours to12 hours,while there were no differences 24 hours later. Conclusion The results indicate that the nanostructured titanium surface has yielded the best biological effects for cell adhesion and spreading.