长期使用罗格列酮对去势大鼠骨小梁的影响

Effect of long-term administration of rosiglitazone on bone trabecular in the bilaterally ovariectomized rats

目的研究长期使用罗格列酮对去势大鼠骨小梁的影响。方法20只Wistar大鼠双侧卵巢切除,随机分成对照组、给药组,每组10只。给药组每只大鼠给予马来酸罗格列酮0.70 mg以生理盐水溶解灌胃,对照组以相同体积生理盐水灌胃。每组分别在给药4周、8周后随机抽取5只大鼠,使用micro-CT分别测定股骨、胫骨和椎骨骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)。结果给药4周末,同对照组相比,给药组股骨、胫骨和椎骨骨小梁数目分别减少了26%(P=0.005)、30%(P=0.014)和23%(P=0.002),骨小梁厚度分别减少了35%(P=0.018)、28%(p=0.002)和49%(P=0.000),骨小梁分离度分别增加了18%(P=0.018)、39%(P=0.003)和34%(P=0.002);给药8周末,同对照组相比,给药组股骨、胫骨和椎骨骨小梁数目分别减少了21%(P=0.002)、28%(P=0.000)和22%(P=0.001),骨小梁厚度分别减少了35%(P=0.009)、38%(P=0.027)和49%(P=0.000v),骨小梁分离度分别增加了17%(P=0.003)、39%(P=0.000)和33%(P=0.021)。结论双侧卵巢切除大鼠给予罗格列酮后,骨小梁受损,但骨小梁受损没有随给药时间延长而进一步加重。

Objective To study the effect of rosiglitazone with different time duration of administration on the trabecular in the bilateral ovariectomized （OVX） rats. Methods In this study twenty skeletally mature Wistar rats were subjected to bilateral ovariectomy, randomly assigned into one （n = 10） of the two groups： the control group （C group）, and drug administration group （A group）. Each rat in the A group was given 0.70 mg rosiglitazone per day intra-gastrically for eight weeks, while rats in the C group received normal saline. At the end of 4 weeks and 8 weeks, five OVX rats were selected randomly each time to sacrifice. The femur, tibia and lumbar of each animal were used for three-dimensional measurement by micro-computerized tomography （micro-CT）, including the trabecular number （Tb. N）, trabecular thickness （Tb. Th ） and trabecular separation （Th. Sp）. Results In rats Of A group, the trabecular number of femur, tibia and lumbar declined 21% （P =0.002）, 28% （P =0. 000） and 22% （P =0.001 ）, respectively, while the trabecular thickness of femur, tibia and lumbar declined 35% （ P = 0. 018 ）, 28% （ P = 0.002 ） and 49% （P = 0. 000 ）, respectively, compared to those of C group after 4 weeks. In addition, the trabecular separation of femur, tibia and lumbar increased 18% （ P = 0. 018 ）, 39% （ P = 0. 003 ） and 34% （ P = 0. 002）, respectively, during this period. Similarly, in rats of A group, the trabecular number of femur, tibia and lumbar declined 21% （P =0.002）, 28% （P =0.000） and 22% （P =0.001 ）, respectively; the trabecular thickness of femur, tibia and lumbar declined 35% （ P = 0. 009 ）, 38% （ P = 0.027 ） and 49% （P=0. 000）, respectively; the trabecular separation of femur, tibia and lumbar increased 17% （P = 0.003）, 39% （P = 0. 000） and 33% （P = 0. 021 ）, respectively, compared to those in C group after 8 weeks. Conclusion The use of rosiglitazone destroys the bone. But the degree of bone loss induced by rosiglitazone does not correlate with the prolonged time duration of the drug administration.