姜黄素协同β-榄香烯诱导涎腺腺样囊性癌细胞株SACC-83凋亡的研究

Study on the apoptosis of adenoid cystic carcinoma cell Line SACC-83 induced by curcumin and β-elemene

[目的]研究姜黄素协同β-榄香烯诱导体外培养涎腺腺样囊性癌细胞株SACC-83凋亡的情况。[方法]应用MTT测定法、流式细胞术和透射电镜观察等方法观察姜黄素协同β-榄香烯(浓度比1∶1)诱导SACC-83细胞凋亡及其与G2/m期阻滞关系。[结果](1)应用MTT测定法,姜黄素协同β-榄香烯(浓度比1∶1)作用SACC-83细胞24 h后,其抑制率有明显协同效应。(2)流式细胞术分析,10μg/mL姜黄素与10μg/mLβ-榄香烯联合作用SACC-83细胞24 h后诱导SACC-83细胞发生凋亡,并将细胞周期阻滞于G2/m期。(3)透射电镜观察,协同用药作用SACC-83细胞24 h后,出现明显细胞凋亡特征。[结论]姜黄素和β-榄香烯(浓度比1∶1)对SACC-83细胞的抑制有协同作用,并诱导其产生凋亡,使其细胞周期阻滞于G2/m期。

[ Objective] To study the apoptosis of salivary adenoid cystic carcinoma cell line SACC -83 induced by curcumin and β -elemene. [ Methods] Curcumin and β -elemene （concentration 1：1 ）induced arrest of cell G2/m phase and apoptosis of SACC - 83 cells were determined, which were checked by MTT color assay and observed with flow cytometry and electronmicroscope. [ Results ] Using MTT test, the synergistic effect of cureumin and β- elemene could inhibit SACC - 83 cells effectively after 24 hours, which did SACC - 83 cell growth in a close - dependent manner. SACC - 83 cells were induced to apoptosis in 24 hours by 10 μg/mL curcumin and 10μg/mL β - elemene. SACC - 83 cells were blocked during G2/m phase. Cells undergoing apoptosis exhibited the following typical features： an apparent apoptotic peak during section of cell cycle with flow cytometry. 10 μg/mL curcumin and 10 μg/mL β-elemene（ with 24 hours）inhibited SACC -83 ceils. The trypical uLtra - structral changes of apoptosis observed by electron - microscope was ehromatin＇ s cling to periphery of the shrunken nuclear. [ Conclusions] The synergistic effect of curcumin and β - elemene （ concentration 1 ： 1 ） could inhibit SACC - 83 cells and induce apoptosis. Arrest of G2/m phase might cause apoptosis of SACC - 83 cells.