日粮硒0.2mg·kg^(-1)水平对鸡肝组织中GPx活性影响的Meta分析

Effect of 0.2 mg·kg^(-1) Selenium in Broiler Diets on the Activity of GPx in Hepatic Tissue:a Meta-analysis

为了整合前人的研究,进行综合判断,从循证医学角度对硒与GPx关系进行再认识。本研究通过检索多个数据库及相关资源,利用Meta分析的统计方法,将前人具有共同研究目的相互独立的多个研究结果合并、定量分析,剖析各研究间的差异特征。Meta分析结果表明,对照组与试验组之间鸡肝组织GPx活性差异显著(P<0.01),硒与GPx活性正相关。但Meta分析结果同时也指出,过去的研究中存在夸大或低估硒对GPx活性作用的事实,本研究中在日粮硒0.2 mg.kg-1水平时,试验组鸡肝组织GPx活性比对照组提高26.79μg.mg-1左右是具有统计学意义的。经典研究方法在鉴别硒与GPx关系方面所得出的结果总的倾向是可信的,但是具体到相关程度的研究则显得不够精确,甚至相互矛盾,这种历史现象也正反映了Meta分析的必要性,也与Meta分析在当今国际兽医学发展中的未来前景符合。

This study was intended to combine previous results of different independent researches and judge them integrally to re-new our knowledge of the co-relationship between selenium and the activity of GPx from the aspect of evidence-based medicine.A Meta-analysis was facilitated,by searching multi-databases and related resources,and performed by combining and quantitative previous multi-results of different independent researches.The different characteristics of each research were checked.The results of Meta-analysis showed that there was a significant difference of GPx activity in chicks′ hepatic tissue between experimental and control groups（P〈0.01） and there was a positive co-relationship between seleniumand the activity of GPx.But the result of Meta-analysis also indicated that there were the facts that the effect of selenium on GPx activity had been overestimated or underestimated in the past,and an extra 26.79 μg·mg-1 of GPx activity in comparison with control group in chick′s hepatic tissue had statistic importance when 0.2 mg·kg-1 selenium was supplied in diets.The results of the co-relationship between selenium and the activity of GPx generated by classical methods were credible,while it was unprecious to predict the degree of the co-relationship and contrary to each other sometime.This historical phenomena just right reflected the necessary of Meta-analysis and this is in keeping with the development of veterinary medicine.