他莫昔芬对人肝癌HepG2细胞增殖和Smad7表达的影响

Effect of tamoxifen on the Smad 7 expression and proliferation of human hepatoellular carcinoma HepG2 cells

目的研究他莫昔芬(TAM)对人肝癌HepG2细胞增殖的影响,探讨其作用机制。方法将他莫昔芬配制为0、7.5、l5、30μmol/L,与人肝癌HepG2细胞培养24、48、72h,用四甲基偶氮唑蓝(MTT)法测定人肝癌HepG2细胞的抑制率;免疫组化染色观察人肝癌HepG2细胞Smad7蛋白的阳性表达。结果人肝癌HepG2细胞增殖随他莫昔芬浓度增加和处理时间的延长,细胞抑制率明显增加,细胞浆内Smad7蛋白阳性表达率明显降低,与时间和浓度呈负相关。结论他莫昔芬可抑制肝癌细胞增殖,可能与下调Smad7蛋白的表达有关。

Objective To study the effect of tamoxifen on proliferation of human hepatoellular carcinoma HepG2 cells.Methods The HepG2 cells were treated with tamoxifen at concentrations of 0,7.5,15 and 30 μmol/L.The cells were further cultured for 24,48 and 72 h.The suppression rate of HepG2 cells was determined with MTT.The Smad7 expressive rate of HepG2 cells was examined by immunohistochemistry.Results Tamoxifen inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner.Tamoxifen suppressed expression of Smad7 of HepG2 cells also in a dose-and time-dependent manner.Conclusions Tamoxifen may suppress human hepatocelular carcinoma HepG2 cell proliferation by down-regulating Smad 7 expressions.