手术创伤动员血管内皮祖细胞入血与移植瘤生长的关系

Correlation of tumor growth and endothelial progenitor cells entering blood induced by surgical injury

目的研究手术创伤动员荷瘤裸鼠血管内皮祖细胞(EPC)入血与实体瘤生长的关系。方法将42只荷瘤裸鼠随机分为非手术1d组、单纯麻醉组、手术创伤组(术后24h、48h、72h、30d组)及非手术30d组,每组6只。单纯麻醉组24h后取血及获取移植瘤组织,手术创伤组分别在术后24h、48h、72h、30d取血及获取移植瘤组织。流式细胞仪检测各组外周血EPC百分比;ELISA检测血清血管内皮生长因子(VEGF)水平;免疫组织化学法检测移植瘤组织VEGF表达及微血管密度(MVD)。结果术后24h、48h、72h组EPC百分比与非手术1d组比较,差异均有统计学意义(P<0.05);术后24h组、48h组、72h组、单纯麻醉组VEGF水平与非手术1d组比较,差异有统计学意义(P<0.05);各组MVD差异无统计学意义(P>0.05)。Pearson相关性分析显示,血清VEGF水平与外周血EPC百分比呈正相关(r=0.6956,P<0.01),外周血EPC百分比与MVD无相关性(r=0.2214,P>0.05),血清VEGF水平与MVD也无相关性(r=0.2249,P>0.05)。结论手术创伤对移植瘤生长无明显促进作用。

Objective To explore the correlation of tumor growth and endothelial progenitor cells （EPC） entering blood induced by surgical injury in tumor bearing nude mice. Methods Forty-two tumor bearing nude mice were randomly divided into seven groups （n=6）： non-surgical injury groups （1 d and 30 d）, anesthetic group, surgical injury groups （24 h, 48 h, 72 h and 30 d after surgery）. Blood samples and xenograft tumor tissues were taken from anesthetic group 24 h after anaesthesia and surgical injury groups 24 h, 48 h, 72 h and 30 d after surgery. EPC levels in peripheral blood were measured by flow cytometry, serum VEGF levels were determined by ELISA, microvessel density （MVD） and expression of VEGF were detected by immunohistochemistry. Results The levels of EPC in 24 h post-surgery group, 48 h post-surgery group and 72 h post-surgery group were significantly higher than that in non-surgical injury 1 d group （P〈0.05）. The levels of VEGF in 24 h post-surgery group, 48 h post-surgery group, 72 h post-surgery group and anesthetic group were significantly higher than that in non-surgical injury 1 d group （P〈0.05）. There was no significant difference in MVD among groups （P〉0.05）. Pearson correlation analysis revealed that serum VEGF levels were related to EPC levels in peripheral blood （r=0.695 6, P〈0.01）, while EPC levels in peripheral blood were not related to MVD （r=0.221 4, P〉0.05）, and serum VEGF levels had no correlation with MVD （r=0.224 9, P〉0.05）. Conclusion Surgical injury has no obvious influence on xenograft tumor growth.