防石酸奶与市售酸奶降低大鼠尿草酸排泄量的效果比较

Effects of kidney calcium oxalate calculus resistant acidophilus milk versus commercially available sacidophilus milk on urinary oxalate excretion in rats

目的比较自制结石预防型酸奶(防石酸奶)与某市售品牌酸奶对大鼠尿草酸排泄量的影响。方法50只SD大鼠随机分为5组(n=10),连续20d分别每日灌喂4mL自制防石酸奶(防石酸奶组)、某市售品牌酸奶(市售品牌酸奶组)、灭菌防石酸奶(灭菌防石酸奶组)、灭菌某市售品牌酸奶(灭菌市售品牌酸奶组)和去离子水(空白对照组),灌喂前1天及灌喂期间每隔4天测量大鼠体质量及24h尿草酸排泄量。结果灌喂期间,各组大鼠体质量和24h尿草酸排泄量均逐渐增加;灭菌防石酸奶组、灭菌市售品牌酸奶组和空白对照组尿草酸排泄量差异无统计学意义(P>0.05);防石酸奶组和市售品牌酸奶组尿草酸排泄量的增加趋势明显小于其他三组,其分别于灌喂8d和12d后显著小于灭菌防石酸奶组和灭菌市售品牌酸奶组(P<0.05);防石酸奶组尿草酸排泄量于灌喂16d后小于市售品牌酸奶组(P<0.05);各组大鼠体质量与24h尿草酸排泄量呈正相关(r=0.97～0.99,P<0.01)。结论自制防石酸奶与某市售品牌酸奶均可降低大鼠尿草酸排泄量,前者效果显著优于后者。

Objective To compare the effects of kidney calcium oxalate calculus resistant acidophilus milk （KCOCRAM） versus commercially available acidophilus milk （CAAM） on urinary oxalate excretion in rats. Methods Fifty SD rats were randomly divided into 5 groups （n=10）, and were bred with KCOCRAM （KCOCRAM group）, CAAM （CAAM group）, sterilized KCOCRAM （sterilized KCOCRAM group）, sterilized CAAM （sterilized CAAM group） and water （blank control group）, respectively for a consecutive 20 d, with 4 mL/d for each rat. The data of body weight and 24 h urinary oxalate excretion volume were obtained 1 d before breeding and every 4 d during breeding. Results During breeding, body weight and 24 h urinary oxalate excretion volume of each group increased with time. There was no significant difference in urinary oxalate excretion volume among sterilized KCOCRAM group, sterilized CAAM group and blank control group （P〉0.05）. The increase tendency of KCOCRAM group and CAAM group was weaker than that of the other three groups. The urinary oxalate excretion volume was significantly lower in KCOCRAM group than that in sterilized KCOCRAM group from 8 d after breeding, and that was significantly lower in CAAM group than that in sterilized CAAM group from 12 d after breeding （P〈0.05）. The urinary oxalate excretion volume was significantly lower in KCOCRAM group than that in CAAM group from 16 d after breeding （P〈0.05）. There was a positive correlationship between body weight and 24 h urinary oxalate excretion volume in each group （r=0.97-0.99, P〈0.01）. Conclusion Both KCOCRAM and CAAM can reduce urinary oxalate excretion in rats, and the former has a more favourable effect.