姜黄素对前列腺癌细胞C—erbB-2，Bax和Survivin表达的影响

Effect of curcumin on the expression of C-erbB-2, Survivin and Bax in prostatic cancer cell （ DU145 ）

目的探讨姜黄素对前列腺癌DU145细胞株C—erbB-2，Survivin和Bax的影响。方法利用细胞培养和SP免疫组织化学技术观察了前列腺癌细胞C—erbB-2，Survivin和Bax表达及姜黄素对它们的干预作用。结果对照组DU145细胞质中Survivin和C-erbB-2有较高表达，Bax表达很弱。姜黄素加入（浓度50μmol／m）后培养6h，12h，24h和48h，DU145细胞中Survivin，C—erbB-2表达逐渐减弱，而Bax逐渐增加，12—24h达高峰。结论姜黄素能够上调促凋亡基因Bax表达和下调Survivin和C—erbB-2表达，姜黄素抗肿瘤的途径之一干扰DU145细胞基因蛋白表达。

Objective To explore the effects of curcumin on the expression of C-erbB-2, Survivin and Bax in prost cancer cell. Methods The effects of curcumin on the expression of C-erbB-2, Suevivin and Bax were observed by SP immnohistocherminstry and cell cultivate methods. Results The expression of Survivin and C-erbB-2 in contrast group were strong, but the expression of Bax was very weak. After the curcumin were incubated with culture medium for 6h, 12h, 24h, 48h, the expression of C-erbB-2 and Survivin were gradually decreased in DU145 cells, while the expression of Bax was increased and reached highest at the time point of 12 -24h. Conclusion The curcumin can reduce the expression of C-erbB-2 and surviving, and increase expression of Bax. The curcumin may exert antitumor activity by interacting with DU145 cells.