背角无齿蚌不同组织的基因组DNA甲基化分析

Analysis of the methylation in genome DNA from different tissues of Anodonta woodiana

用甲基化敏感扩增多态性(MSAP)对背角无齿蚌(Anodonta woodiana)腮、唇瓣、闭壳肌、外套膜和斧足五个组织基因组DNACCGG区域的甲基化水平进行了分析。结果表明,背角无齿蚌腮基因组DNA甲基化比例为47.9%,唇瓣甲基化比例为35.5%,闭壳肌甲基化比例为50%,外套膜甲基化比例为46.3%,斧足甲基化比例为56%;基因组中CCGG区域存在甲基化现象。不同组合甲基化比例不同说明该区域甲基化可能参与到基因的调控中。通过比较不同地点的采集的背角无齿蚌,发现污染严重地区(太湖三山岛水域)采集到的样品和非污染地区(南泉养殖水域)采集的蚌样甲基化区域略有变化。其中的相关性有待进一步的研究。

In this study, methylation sensitive amplified polymorphism （MSAP） was applied to analyze the methylation pattern in CCGG sites of the genome in five tissues （gill, labial palp, adductor muscle, mantle and foot） of swan mussels （Anodonta woodiana）. The results showed that methylation ratios of the gills, labial palps, adductor muscle, mantle, and foot were 47.9%, 35.5%, 50%, 46.3% and 56%, respectively. Such differences among tissues suggested that methylation may be involved in the regulation of the gene in Anodonta woodiana. The comparison of the swan mussels from different habitats suggested there were some variations in methylated gene loci between those from polluted environment （Sanshandao area of the Taihu Lake） and aquacultural water （Nanquan aquaculture base of Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences）. Further studies are needed to investigate the oossible mechanism for the aforementioned methvlation variations.