松仁油对小鼠脂肪组织中脂代谢相关基因表达的影响

EFFECT OF PINE-SEED OIL ON LIPID METABOLISM-RELATED GENE EXPRESSION IN ADIPOSE TISSUE OF MICE

目的探讨松仁油对脂代谢相关基因表达的影响。方法分别以基础饲料、高脂饲料(20%猪油和80%基础饲料HF)及高脂饲料加松仁油(20%松仁油和80%高脂饲料SHF)饲喂小鼠,记录体重调整饲喂量,于45d和90d时取睾周脂肪,以RT-PCR法测定酰基CoA合成酶(ACS)、脂肪酸转运蛋白(FATP)、激素敏感脂肪酶(HSL)、过氧化物酶体增殖剂激活受体-γ(PPAR-γ)mRNA的表达水平。结果45d时,SHF组与基础组比,ACSmRNA、FATPmRNA、HSLmRNA和PPAR-γmRNA的表达水平分别增加了24.2%、8.8%、10.9%、21.6%。90d时,SHF组与基础组比,ACSmRNA、FATPmRNA、HSLmRNA和PPAR-γmRNA的表达水平分别增加了19.6%、25.7%、60.1%、13.1%;HF组与基础组比ACSmRNA、FATPmRNA、HSLmRNA和PPAR-γmRNA的表达水平分别增加了27.9%、41.7%、51.1%、23.9%。结论松仁油在调节脂质代谢方面具有积极的意义。

Objective To study the influence of pine-seed oil on lipid metabolism-related gene expression. Method The mice were fed by basic food,high fat food （80% basic food added 20% lard, HF） and HF added pine-seed oil （80% high fat food added 20% pine-seed oil, SHF）. At 45 d and 90 d, the ACS mRNA, FATP mRNA, HSL mRNA and PPAR mRNA of adipose tissue were measured respectively by RT-PCR. Results At 45 d, compared with the basic group, ACS mRNA, FATP mRNA, HSL mRNA and PPAR-γ mRNA expression level in SHF group increased by 24.2%, 8.8%, 10.9%, 21.6% respectively,and at 90 d,increased by 19.6%, 25.7%, 60.1%, 13.1% respectirely. Compared with the basic group,ACS mRNA, FATP mRNA, HSL mRNA and PPAR-γ mRNA expression level in HF group increased by 27.9%, 41.7%, 51.1%, 23.9% respectively. Conclusion Pine-seed oil is significantly effective in regulation of lipid metabolism.