好氧颗粒污泥的培养及其降解硝基苯的活性

Cultivation of Aerobic Granular Sludge and Characterization of Nitrobenzene-Degrading

采用三角瓶在摇床上好氧振荡的方法,用硝基苯(nitrobenzene,NB)废水处理厂的好氧污泥驯化培养能够降解NB的混合菌群,发现在此培养过程中,微生物菌群形成颗粒化(颗粒污泥),采用此颗粒污泥(混合菌群)进行降解NB的研究.结果表明,该混合菌群在以NB为唯一碳源和氮源的情况下降解NB的效果最好,该混合菌群降解NB时最适宜的温度为28℃,能够适宜于pH 9.0以下的弱碱性环境,且最佳的pH值为7.0,当NB的起始浓度为600 mg.L-1时,混合菌群适应期较短,在6 h以下,混合菌群在24 h内能够完全降解NB,降解速率最大,达到28.8 mg.(L.h)-1,由此表明,好氧颗粒污泥法用于含硝基苯类化工废水的处理是一种新的尝试,具有实际应用价值.

The highly efficient nitrobenzene degrading aerobic mixed microbe was cultivated from activated sludge originated from Nanjing chemical plant and it was found that aerobic granular sludge could be formed through the flask trial.The nitrobenzene-degrading experiment was conducted with the obtained aerobic granular sludge.Results showed that the mixed microbe could utilized nitrobenzene as the sole source of carbon and nitrogen for its growth.The optimum growth conditions for the bio-degradation of nitrobenzene were pH 7.0 and 28℃.The adaptive period was no more than 6 hours and its degrading efficiency was highest when the initiative concentration of nitrobenzene was 600 mg·L^-1.The mixed microbe could completely degrade nitrobenzene（600 mg·L^-1） within 24 hours and the maximum degradation rate of nitrobenzene was 28.8 mg·（L·h）^-1.It will be possible that aerobic granular sludge can be used to degrade the organics such as nitrobenzene contained in the industrial waste water.