象山地产海产品副溶血性弧菌污染状况研究

Investigation on Contamination of Vibrio Parahaemolyticus in Xiangshan Native Marine Products

目的了解象山地产海产品中副溶血性弧菌污染状况及其动态变化,探讨其与食物中毒的相关性,为食品安全预警提供监测数据。方法按不同季节分批从本地水产销售市场采集鱼类、甲壳类、贝类样品,采用MPN法进行副溶血性弧菌的定量检测,并对阳性菌株进行血清学分型及毒力基因检测。结果全年海产品副溶血性弧菌平均检出率为54.2%,最高含量≥24000MPN/100g;检出率及污染量以第3季度为最高;各类海产品中以贝类受污染程度最严重;分离到的副溶血性弧菌血清型分布以O11、O4、O5为主,其中3株携带TDH基因。结论象山地产海产品中副溶血性弧菌污染较严重,具有明显的季节性,与本地食物中毒的发生相一致,是引起食源性疾病的主要危险因素,应予以重视。

Objective To understand the vibrio parahaemolyticus contamination in Xiangshan native marine products and explore the relationship between marine products contamination and food poisoning. Methods Fish, crustacean and shellfish were collected from local marine products market during different seasons. Then MPN method was applied to detect vibrio parahaemolyticus quantitatively. To those positive stains additional serotyping and virulence gene detection were carried out. Results The average detected rate of vibrio parahaemolytieus was 54.2% all the year around, with a maximal content ≥24000MPN/100g. Both the detected rate and contamination level turned up highest in the third quarter of a year while the degree of pollution in the shellfish was the heaviest. Additionally, the distribution of seretypes was primarily concentrated in O11/O4/O5, and three of them carried with tdh gene. Conclusion Xiangshan native marine products are contaminated severely with vibrio parahaemolytieus and shows an obvious seasonal pattern, which is identical with the season of local food poisoning. Therefore, vibrio parahaemolyticus contamination should be paid more attention to as a primary risk factor of foodborne diseases.