肝细胞不典型增生细胞核DNA含量与甲胎蛋白癌胚抗原及铁蛋白表达的关系

THE RELATIONSHIP BETWEEN NUCLEAR DNACONTENT AND EXPRESSION OF AFP CEA ANDFERRITIN IN LIVER CELL DYSPLASIA

目的探讨肝细胞不典型增生细胞核ＤＮＡ含量与甲胎蛋白、癌胚抗原及铁蛋白３种肿瘤标志表达之间的关系。方法采用免疫组织化学ＰＡＰ法及显微分光光度计检测２２例肝细胞不典型增生甲胎蛋白、癌胚抗原和铁蛋白的表达情况和细胞核ＤＮＡ含量。结果肝细胞不典型增生的甲胎蛋白、癌胚抗原表达与非不典型增生和肝癌细胞不同，而铁蛋白的表达在三者之间无明显差别。细胞核ＤＮＡ含量及分布介于癌细胞与非不典型增生细胞之间（Ｐ＜０．０５）。结论肝细胞不典型增生细胞核ＤＮＡ含量的变化与甲胎蛋白、癌胚抗原的表达存在一定的关系，提示肝细胞不典型增生是一类增生性病变，其中含非整倍体较多的可能是一种癌前细胞。

Objective To assess the relationship between nuclear DNA content and expression of three tumor markers in liver cell dysplasia.Methods AFP,CEA , Ferritin were studied by immunohistochemical Method in 22 cases of liver cell dysplasia (LCD).Nuclear DNA content was determined by using microspectrophotometry in 17 of all 22 patients.Results Showing that LCD was neither like non-LCD nor like hepatocellular carcinoma(HCC)in the expression of the three tumormarkers,and LCD was situated between HCC and non-LCD in the mean value of DNA content and the distribution of DNA ploidy(P<0.05).Conclusion Interior relationship between nuclearDNA content and the expression of the tumor markers was found in the cases of LCD ,suggesting that LCD is a group of proliferating cells, and those with a relatively high percentage of aneuploid cells might be precancerous cells.