摘要
目的研究紫金胶囊对Ｄ氨基半乳糖（ＤＧｌａＮ）致大鼠肝损伤的保护作用．方法Ｗｉｓｔａｒ大鼠造型：ＤＧｌａＮ（０８ｇ／ｋｇ），ｉｐ，１次／ｄ，连续２ｄ；生理盐水１０ｍｌ／ｋｇ，ｉｇ，２次／ｄ，连续３ｄ．设空白组，单纯中药组（１４１ｇ／ｋｇ），联苯双酯（４０５ｍｇ／ｋｇ）阳性对照组，紫金胶囊低、中、高剂量组（２８、１４１、２２６ｇ／ｋｇ）．自动生化分析仪上检测血清的总胆红素含量、凝血酶原时间、总蛋白含量、白蛋白含量及ＡＬＴ活性，组间对比ｔ检验．观察形态结构，作肝小叶百分比、肝细胞索百分比、存活肝细胞核和慢性炎细胞核的形态计量，组间对比ｑ检验或ｔ检验．结果紫金胶囊明显降低血清总胆红素含量（μｍｏｌ／Ｌ，从５７６±４９到７２±０９以下），缩短凝血酶原时间（ｓ，从１３８５±１２５到１０４６±３４以下），升高血清总蛋白含量（ｇ／Ｌ，从４９２±２２到５７２±２１以上），升高血清白蛋白含量（ｇ／Ｌ，从３００±１６到３９７±１７以上）及降低血清ＡＬＴ活性（μｍｏｌ／ｍｉｎ，从３３７４±９５３到８９３±１５３以下）；紫金胶囊明显降低肝小叶百分比（从７３７到７１２以下）和降低肝?
IM To confirm the effect of Zijin capsule in preventing rat liver injury induced with Dgalactasamine hydrochloride.METHODS Rat liver injury models were induced with Dgalactasamine hydrochloride (08g/kg) ip daily for two days. Compared with biphenyl diocarboxylate (405mg/kg) by liver functions and morphometry on the models, Zijin capsule was given at three doses (28, 141 and 226g/kg) intragastrically, the others were same as those in the model group. NS was given to the normal group. The liver functions were examined with biochemistry and expressed as ±s. The liver morphometry was observed under microscope and expressed as X-i for hepatic lobule ratio or hepatic lamina ratio, as ±s for normal hepatic nuclear and infiltrated inflammatory cells.RESULTS Zijin capsule could reduce the TBil from 576μmol/L±49μmol/L to 72μmol/L±09μmol/L or less, Tcoag from 1385s±125s to 1046s±34s or less and ALT from 3374±953 to 893±153μmol/min or less, and maintain the content of total protein from 492g/L±22g/L to 572g/L±21g/L or more or albumin from 300g/L±16g/L to 397g/L±17g/L or more in serum. The capsule could reduce the ratio of hepatic lobule from 737 to 712 or less or hepatic lamina from 588 to 514 or less, maintained the number of hepatocytes from 2398±626 to 3060±566 or more, and minimized the infiltrated inflammatory cells from 3391±502 to 1007±232 or less.CONCLUSION Zijin capsule can protect against the rat liver injury induced with Dgalactasamine hydrochloride.
