玉屏风汤剂多糖乙酰化物GC特征图谱研究

GC Fingerprint Analysis on Acetylate of Polysaccharides from Yupingfeng Decoction

目的:建立玉屏风汤剂多糖乙酰化物的GC特征图谱分析方法,拟定其特征图谱指标成分,并对汤剂多糖的组成、相对比例及稳定性进行分析。方法:玉屏风汤剂经提取和纯化得玉屏风多糖粗品,多糖粗品经水解及乙酰化后采用GC法分析,色谱条件:HP-5柱;柱温采用程序升温,起始160℃,5℃·min^(-1)升至250℃;气化温度为250℃;检测器温度为280℃;流速为0.9 ml·min^(-1)。结果:9批玉屏风汤剂中共标示出5个共有峰,分别为鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖。结论:方法准确可靠,为玉屏风汤剂的研究提供了一定参考。

Objective： To establish the GC method of fingerprint analysis on Aeetylate Polysaeeharides from Yupingfeng Decoction, work out the characteristic fingerprint,and analyze the monosaccharide composition,ratio and stability of polysaceharide. Method： To extract and purify the Polysaccharides from Yupingfeng Decoction. the purified Polysaecharides were Hydrolysised and aeetylated,and it was analysed by GC with HP-5 ;chromatographic column at heating-up;initial temperature at 160 ℃, temperature up to 250 ℃at 5 ℃·min^- 1 ; the temperatures of sample vent and FID were 250 ℃ and 280 ℃ ;the flow rate was 0.9 ml·min^-1. Result： Five common peaks were separated in 9 batches of Polysaccharides from Yupingfeng Decoction, the characteristic peaks were rhamnose, arabinose, mannitose, glucose and galactose. Conclusion： The method is reliable, accurate and provides a reference for study of Yupingfeng Decoction.