鞑靼荞麦离体再生体系的建立

In vitro Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.)

研究了苗龄、外植体、激素配比对鞑靼荞麦(Fagopyrum tataricum Gaertn.)离体培养的影响,初步建立了鞑靼荞麦离体再生体系.结果表明,鞑靼荞麦离体再生的最佳取材时间为苗龄6～8d;诱导愈伤组织的最适培养基为MS+2.0mg/L2,4-D+1.5mg/L6-BA,子叶诱愈率达75%左右,下胚轴诱愈率可高达86.62%;愈伤组织分化的最适培养基为MS+0.1mg/L IAA+2.0mg/L6-BA+1.0mg/L KT+0.5mg/L TDZ,来自下胚轴愈伤组织的分化率可达9.52%.下胚轴的诱愈率与分化率均高于子叶,更适于离体再生培养.培养基中加入AgNO3后,能有效降低褐化率.生根最适培养基为含有0.5mg/L NAA的1/2MS培养基,生根率在50%左右.TDZ在诱导鞑靼荞麦的愈伤组织分化出芽的过程中起到明显的促进作用,可提高分化率约20%.

The effects of seedling age,hypocotyl and cotyledon as explants,and proportions of several growth regulators were tested in tissue culture of Fagopyrum tataricum Gaertn. for establishing its in vitro regeneration system. The results showed that the best seedling age for callus induction was 6 to 8 days. On the MS medium containing 2.0 mg/L 2,4-D and 1.5 mg/L 6-BA,the induction rate of calli from hypocotyls was up to 86.62%,while that from cotyledons was about 75%. The suitable shooting medium was the MS medium＋0.1 mg/L IAA＋2.0 mg/L 6-BA＋I.0 mg/L KT＋0.5 mg/L TDZ （thidiazuron）, and the shooting rate from hypocotyls was 9.52%. The callus induction and shooting rates were higher from hypocotyls than from cotyledons. Browning was reduced by the medium mixed with 3.5 mg/L AgNO3. Half strength MS supplemented with 0.5 mg/L NAA was the best for rooting with a rate around 50% after 30 days＇ culture. TDZ could accelerate shoot differentiation distinctively, improving the shooting rate by nearly 20%. Fig 3, Tab 2, Ref23