嗜热真菌木聚糖酶与稀酸联合水解甜菜渣生产L-阿拉伯糖的初步研究

Characterization of Sugar Beet Pulp Hydrolysate by Xylanase and Diluted Acid for L-Arabinose Production

通过嗜热真菌DSM 10635固体发酵生产木聚糖酶,探讨了利用该木聚糖酶-稀酸联合水解甜菜渣生产稀少五碳糖的方法,并对水解产物进行了定量分析.研究发现:通过木聚糖酶-稀酸水解甜菜渣可得到稀少单糖,主要有阿拉伯糖、葡萄糖、木糖等.HPLC结果显示:甜菜渣的水解产物中L-阿拉伯糖含量占全部单糖含量的48.4%(质量分数).实验结果为进一步研究L-阿拉伯糖的工业生产和精制奠定了基础.

Thermostable xylanase was produced by solid substrate fermentation of Thermomyces lanuginosus DSM10635 in this study.The hydrolysates of sugar beet pulp by xylanase and diluted acid were analyzed by HPLC.The results showed that a few valuable rare saccharides such as the L-arabinose,xylose,etc can be produced from sugar beet pulp.The concentration of L-arabinose in total monosaccharide of sugar beet pulp hydrolysate is up to 48.4 %,The method has potential for industrial production of L-arabinose.