摘要
为研制鹿布鲁氏菌病工程疫苗,根据已发表的布鲁氏菌核糖体蛋白L7/L12、外膜蛋白OMP31基因设计两对引物进行扩增,PCR产物连接pGEM-Teasy载体,转化DH5α后测序,并进行基因分析。结果从布鲁氏菌疫苗株中克隆出L7/L12和OMP312个目的基因,同源分析L7/L12达97.1%,OMP31达100%,免疫原性分析可作为免疫优势抗原。
To develop engineered vaccine of deer Brucella,two pairs of primers were designed to amplify the gene according to the pub-lished Brucella ribosomal L7/L12 and OMP31 gene.PCR products were ligated into pGEM-T easy vector,then transformed into compe-tent DH5αfor sequencing and analyzing these genes.Two target genes,L7/L12 and OMP31,were cloned from the Brucella vaccine strain.L7/L12 had 97.1%,and OMP31 had 100% according to the homology analysis.Based on the immunogenicity analysis we knowthat it can be used as a immune advantage antigen.
出处
《特产研究》
2009年第4期5-8,共4页
Special Wild Economic Animal and Plant Research
基金
国家科技支撑计划(2006BAD06B06)
