牛卵母细胞显微操作机械去核方法的比较

Comparison of micromanipulation mechanical enucleation methods of bovine oocytes

分别采用挤压去核法、盲吸去核法和点击去核法对延边黄牛卵母细胞进行去核操作,在操作时间、成功率、去核率及重组胚后期发育等方面进行比较.结果表明,去核率上,盲吸法低于其他2种方法;去核成功率上,挤压法和点压法高于盲吸法;操作耗时上,挤压法和盲吸法耗时较短;3种方法所构建的重组胚卵裂率以及囊胚发育率均差异不显著.因此,挤压去核法是一种较适合延边黄牛卵母细胞去核的有效方法.

Oocytes of Yanbian Yellow Cattle were enucleated by methods of squeezing enucleation, blind enucleation and point-hitting enucleation, then compared in operating time, achievement rate, enucleation rate and proportion of reconstituted embryos . The results showed that blind enucleation was lower than the other two ways in enucleation rate. Higher success rate were obtained using squeezing enucleation and point-hitting enucleation. The shorter time-consuming methods were squeezing enucleation and blind enucleation; there is no significant difference in cleavage rate and blastocyst rate. Therefore, the methods of squeezing enucleation was a practical enucleation method of Yanbian Yellow Cattle oocytes.