利用光诱导延迟荧光的除草剂光合代谢损伤的快速在位测定

Rapid Determination of Damages of Photosynthetic Metabolism Caused by Herbicides Using Light-induced Delayed Fluorescence

叶绿体结构与功能以及相应叶片光合作用能力会都会受到除草剂的影响.植物叶绿体光系统II(PSII)被认为是光诱导延迟荧光(DF)的最主要的产生位点.以京黄3号大豆品种为测试植物,利用自行研制的便携式延迟荧光检测系统,研究了三种功能已知除草剂对DF衰减动力学曲线的影响.结果表明,通过解析延迟荧光衰减动力学曲线,发现延迟荧光衰减双指数数理解析式的变化能准确、真实的反应不同除草剂光合电子传递链的作用机制和作用位点.因此,延迟荧光衰减动力学可为除草剂药理分析、作物光合代谢损伤诊断以及新一代除草剂开发提供一实用、无损的新方法.

The structure and function of chloroplast in plant leaves can be affected by herbicide, resulting in the decrease in photosynthetic activity. The photosystem II（PSII） in a plant is considered the primary site where light-induced delayed fluorescence （DF） is produced. With the lamina of soybean （ Glycine max Jinghuang No. 3） as testing plant, we have studied the effects of three different kinds of herbicides on decay kinetics of DF using a homemade portable DF detection system. Current investigations have demonstrated that the analytic equation of DF decay dynamics we proposed here can truly and exactly reflect the mechanism and site about which herbicides inhibit photosynthetic process. Therefore, DF decay dynamics may provide a practical and non-invasive means of pharmacology analysis of herbicides and damage-diagnosis of photosynthetic metabolism. It could be potentially useful for development of new generation herbicides.