摘要
目的研究植物化学药物槲皮素对人结肠癌细胞的生长抑制作用和凋亡诱导效应。方法分别用不同浓度的槲皮素对人结肠癌细胞(Lovo细胞株)进行干预,采用MTT检测细胞增殖,流式细胞术检测细胞凋亡及细胞周期。结果与对照组相比,槲皮素对Lovo细胞具有显著的增殖抑制作用(P<0.05),细胞增殖抑制随浓度增加、时间延长逐渐降低,呈现量-效、时-效关系。槲皮素引起明显的细胞周期阻滞(P<0.05),将细胞周期阻滞在G2/M期。槲皮素呈浓度依赖性诱导细胞凋亡(P<0.05)。结论槲皮素对结肠癌Lovo具有增殖抑制效应及凋亡诱导效应,是一种具有发展潜力的抗结肠癌药物。
Objective To investigate the effect of Quercetin on the proliferation and apoptosis of the colon carcinoma cell line Lovo.Methods The cultured Lovo cells were treated with different concentrations of Quercetin for different time.The proliferation of Lovo cells was detected by MTT method.Cell apoptosis and cell circle were detected by flow cytometry.Results Quercetin inhibited the proliferation of Lovo cells in a concentration-and time dependent manner.Quercetininduced the apoptosis and inhibted the cell circle of Lovo cells in a concentration-dependent manner.Conclusion Quercetin can induce the apoptosis of the colon carcinoma cell line Lovo,reduce the proliferation of Bcl-2,and inhibite the cell circle of colon carcinoma.
出处
《中国实验诊断学》
北大核心
2009年第12期1686-1688,共3页
Chinese Journal of Laboratory Diagnosis
关键词
槲皮素
凋亡
增殖
细胞周期
结肠癌
Quercetin
apoptosis
proliferation
cell circle
colon carcinoma
