实时荧光定量PCR方法检测乳腺癌患者外周血中uPA基因表达水平及其临床意义

Detection of uPA gene expression in breast cancer with realtime PCR and its clinical significance

目的探讨人外周血中尿激酶型纤溶酶原激活物(urokinase-type plasminogen activator,uPA)基因表达水平与乳腺癌临床分期和转移的关系,评价其对乳腺癌辅助诊断及判断预后的临床意义。方法采用实时荧光定量PCR方法检测71例正常人和90例乳腺癌患者外周血中uPA mRNA表达水平。结果97.8%的乳腺癌病人外周血中有uPA基因表达,在正常人外周血中的表达率为40.8%;基因表达水平与临床分期和肿物转移情况相关,P值均<0.05;在患者年龄、肿瘤组织学类型及肿瘤大小分组中,uPA基因表达未发现显著性差异。结论乳腺癌患者外周血中uPA基因表达水平明显增高,并且与肿瘤细胞的转移和病理分期有关。应用实时荧光定量PCR的方法检测外周血中uPA基因表达水平对乳腺癌的辅助诊断及判断预后具有一定的意义。

Objective To investigate the relationship between uPA expression in peripheral blood and its clinical stage and metastatic potential in breast cancer as well as its clinical significance.Methods uPA mRNA was measured by real-time fluorescent quantitative PCR in 71 cases of normal subjects＇ peripheral blood and 90 cases of breast cancer patients＇ peripheral blood.Results The expression of uPA gene was elevated in 97.8% of breast cancer patients and in 40.8% of normal subjects.There was a strong correlation between uPA expression and clinical stage and metastasis（P〈0.05）.No significance was found in uPA expression in the age,pathological type and tumor size.Conclusion The uPA gene is highly expressed in most of the breast cancer,and there is strong correlation to pathological stage and metastasis.Detection of uPA expression in peripheral blood has certain significance for diagnosis and prognosis of breast cancer with real-time fluorescent quantitative PCR.