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NRSE/RE-1 dsRNA诱导小鼠胚胎干细胞分化为NSE阳性的神经元样细胞 被引量：1

Effect of NRSE/RE-1 dsRNA in Inducing Differentiation of Mouse Embryonic Stem Cells into Neuron-like Cells
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摘要 应用电打孔的方法将化学合成的NRSE/RE-1d sRNA转染小鼠胚胎干细胞,在去除LIF的条件下,直接接种培养,观察其分化情况,并对分化结果进行相关检测.结果显示分化细胞呈明显的神经样改变,免疫荧光显示NSE阳性率为(82.3±8.1)%.说明通过转染NRSE/RE-1d sRNA能有效地诱导小鼠胚胎干细胞向神经元细胞分化. Mouse embryonic stem cells were transfected with NRSE / RE-1 dsRNA through the electropo-ration and then cultured in medium without LIF. The differentiated cells were observed and detected by related means. Their morphology were obviously neuron-like alteration and （82.3 ±8.1）% NSE-positive. These results showed that the exogenous NRSE / RE-1dsRNA can efficiently induce ESCs differentiate into neurons in vitro.
作者 李宏图 顾文佳 孟凡彪 马兰兰 庞希宁
机构地区 中国医科大学发育生物学教研室 辽宁省计划生育科学研究院辽宁省生殖医学重点实验室
出处 《生命科学研究》 CAS CSCD 2009年第6期477-481,共5页 Life Science Research
基金 辽宁省自然科学基金资助项目(20062090) 辽宁省教育厅资助项目(2004D227)
关键词 胚胎干细胞 NRSE/RE-1d SRNA 诱导分化 神经元样细胞 embryonic stem cells NRSE/RE-1dsRNA neural induction neuron-like cells
分类号 Q254 [生物学—细胞生物学]
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参考文献12

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二级参考文献25

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共引文献3

同被引文献18

  • 1中华人民共和国科学技术部.关于善待实验动物的指导性意见.2006.09-30
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生命科学研究
2009年 第6期

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