微囊化大鼠胰岛移植物的制备与异种移植

Peraparation of microencapsulated rat islets transplant and xenotransplantation

为降低胰岛移植中排斥反应，用海藻酸钠－聚赖氨酸生物膜制备微囊化大鼠胰岛，体外培养，胰岛素释放试验功能良好，胰岛素释放量与单纯胰岛差异无显著意义；异种移植可成功地纠正糖尿病小鼠的高血糖状态平均达４个月之久。表明：大鼠，胰岛微囊化可在不便用免疫抑制剂的情况下，有效地延长胰岛在糖尿病小鼠体内的生存期。

To circumvent immune destruction of pancratic islet grafts,rat islets were encapsulated in alginate-polylysine -algi nate semipermebale membranes,In vivo studies demonstrated that encapsulated islets secreted insulin in response to glucose challenge for at lesat 4 weeks,which was simliar to that exhibited by free islets,In vivo studies,10 streptozotocin-induced diabetic C57BL/6 mice were transplanted intraperitoneally with 1,000 encapasulated islets each in the absence of immunosuppression.Diabetes was reversed within 3 days , and the animals remained nomoglycemic tinr up to 160 days,with a mean xenograft suwival of 126 days. This was signifi- cantly longer than that by nonencapsulated isleta which functioned for <8 days. Our results clearly indicatc that encapsulation of pancre- atie islets in semipcnmeable membranes can effectively prolong xenugraft survival without immunsuppression in an animal model.