利用成体干细胞体外小口径血管的构建

Construction of Small-Sized Blood Vessel Using SCs in vitro

设计了一套血管生物反应器系统,采用有限元的方法对组织工程小血管托架材料进行了分析,从而开发完成了一套用于构建直径为2 mm的小血管托架;通过收集人的原代骨髓基质干细胞(hBMSCs)和脂肪基质干细胞(ADSCs)进行体外扩增和培养,并选用第3代细胞与聚羟基乙酸酯(PGA)复合后置于血管生物反应器中动态培养;在生物反应器中动态培养4周后,对材料复合物进行取材,分别进行大体观察、HE染色和扫描电镜等指标检测。结果表明:血管色泽明亮,有一定的弹性,细胞分泌的胶原基质排列较规则,免疫组化结果表明血管含有平滑肌弹性肌动蛋白的成分。说明改进的血管生物反应器能模拟血管的力学环境,并能利用人骨髓间充质干细胞和脂肪基质干细胞成功构建组织工程小血管组织。

A set of bioreactor system was designed with the application of finite element methods as an analysis method to construct tissue engineered blood vessels in the diameter of 2 mm.hBMSCs and ADSCs were first collected and further cultivated.The third passage of the cultivated cells were then seeded on the poly（glycolic acid）（PGA） to fabricate the cell-scaffold compound.Subsequently,this compound was subjected to dynamical culturing in the blood vessel bioreactor.Tissue-engineered blood vessels can be constructed by culturing the hBMSCs-PGA and hADSCs-PGA compound in a dynamic condition.After cultered for 4 weeks,the tissues were removed from the bioreactor and subjected to analysis by gross view,HE and SEM（scanning electron microscope）.It was observed that the tissue-engineered blood vessel has a bright color and certain elasticity.The secreted collagen matrix arrayed orderly around the cells and smooth muscle elastic actin can also be detected in the formed tissues.In conclusion,biological conditions of blood vessels can be simulated using the current blood vessel bioreactor system.Using hBMSCs and ADSCs,the construction of the tissue engineered small-sized blood vessels can be successfully achieved in our bioreactor system.