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牛乳铁蛋白ELISA检测方法的研究 被引量：1

Detection of bovine lactoferrin with ELISA
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摘要 本文建立了一种ELISA检测牛乳铁蛋白的方法。主要摸索了ELISA的工作条件,采用方阵滴定法确定抗原包被浓度和一抗稀释浓度,进而确定了二抗工作浓度,通过重复性试验和交叉性试验验证了该法的重现性和特异性。结果表明一抗工作浓度为1∶200000稀释,二抗工作浓度为1∶100000稀释,在此条件下检测牛乳铁蛋白的限度为1.56-100ng/mL,在此范围内,牛乳铁蛋白浓度与OD值之间呈指数相关,其回归方程为y=1.2770×(1.0145-e-0.0338x)(R2=0.9992)。本研究为研制牛乳铁蛋白检测试剂盒奠定了基础。 The method about detection of bovine lactoferrin with ELISA was founded in this study. The protocols of ELISA were performed as follows： the first antibodies were diluted to 1：200 000, the second antibodies were diluted to 1：100 000. The limits of this protocol were 1.56-100 ng/mL. The resulting regression equation was y=1.2770×（1.0145-e-0.0338x）（R2=0.9992）.
作者 王燕平 陆小冬
机构地区 长江大学生命科学学院
出处 《江西食品工业》 2009年第4期28-29,共2页 Jiangxi Food Industry
关键词 牛乳铁蛋白 ELISA 检测 食品工业 Bovine lactoferrin ELISA Detection
分类号 TS252.7 [轻工技术与工程—农产品加工及贮藏工程]
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共引文献43

同被引文献19

  • 1黄耀凌,刘智宏,叶妮,郭筱华.ELISA法检测牛奶中链霉素残留量[J].中国乳品工业,2007,35(2):54-56. 被引量：22
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江西食品工业
2009年 第4期

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