摘要
用乙醇提取板栗花粉中的总黄酮,对提取条件进行优化,最佳提取条件为:提取温度60℃、提取时间1.5h、乙醇浓度80%、液固比10:1,水浴加热提取,在此条件下,用"NaNO2-Al(NO3)3-NaOH"显色法测定板栗花粉中的总黄酮含量为1.68%,采用高效液相色谱法(HLPC)定性分析黄酮物质。
Extraction conditions of total flavonoids from chestnut pollen with ethanol are studied and optimized, the optimal extraction conditions are as follows： temperature of 60℃, time of 1.5h, ethanol content of 80% and ratio of liquid to solid of 10：1, flavonoids are extracted using the water-bath heating, In these conditions, the content of flavonoids in the extracts is 1.68%, using the determination methods of ＂NaNO2-Al（NO3）3-NaOH＂ , flavonoids are qualitative analyzed using high-performance liquid chromatography （HLPC） .
出处
《山东食品发酵》
2009年第3期40-44,共5页
Shandong Food Ferment
关键词
板栗花粉
总黄酮
提取
可见分光光度法
高效液相色谱
chestnut pollen
total flavonoids
extraction
visible spectrophotometry
high-performance liquid chromatography
