摘要
建立了医药中间体1-苯基-3-甲基-吡唑啉酮-5的高效液相色谱定量分析方法。1-苯基-3-甲基-吡唑啉酮-5浓度在10.0-50.0μg.mL-1范围内与峰面积有良好的线性关系,相关系数为0.99999,相对标准偏差为0.63%,平均加标回收率为90.0%-103.0%,最低检出限为0.01μg.mL-1。
A HPLC quantitative analysis method has been developed for the determination of medicine intermediate 1-phenyl-3-methyl-pyrazolone-5.The research method showed that the linear relationship between peak area and sample concentration was satisfactory within the range of 10.0～50.0 μg·mL-1 with coefficient of correlation of 0.99999;the relative standard deviation（RSD） was 0.63%;the average recovery was 90.0%～103.0%;the limit of detection（LOD） was 0.01 μg·mL-1.
出处
《化学与生物工程》
CAS
2009年第12期92-94,共3页
Chemistry & Bioengineering
