利用荧光标记SSR技术鉴定花生F1代杂交种

Identification of Peanut Hybrids Using SSR Marker with Fluorescence Labeled M13-tailed Primer

杂种真实性的鉴定对花生杂交育种和遗传群体构建是非常重要的。利用IRD荧光标记SSR对亲本D145和D089及其F1单株进行分析。结果表明,在可扩增的41对引物中,6对在两亲本中表现多态性,多态性引物比率为14.6%。7个F1单株中,3株表现杂合带型,为真杂种,4株为假杂种,表现母本带型或新带型。根据F2:3家系的网斑病抗性分离表现和农艺性状可知,表现杂合带型的为真杂种,表现母本带型或新带型的为假杂种,与SSR标记鉴定的结果一致。证明应用荧光标记SSR技术鉴定真假杂种是切实可行的。

The identification of true hybrids is important for peanut breeding programs and constructing of genetic populations. In this study, SSR primers labeled with fluorescence M13-tail were used to differentiate between hybrids and non-hybrids. Out of 41 of SSR primer pairs, 6 （14.6%） showed a polymorphism between two parents. On the basis of amplicon patterns combining with agronomic traits of F2：3 families and their resistance to web blotch, F1 plants were true hybrids with heterozygote pattern, and 4 were non-hybrids showing either as same as the pattern of female parent or new pattern. Results showed that SSR marker with fluorescence labeled M13-tailed primer is effective for identification of hybrids in peanut.