SCAR标记在茶树种质资源鉴定中的应用

Application of SCAR Marker in Germplasm Identification of Camellia sinensis

SCAR标记是一种在RAPD技术的基础上发展起来的新型分子标记技术,提高了分子标记辅助选择育种的效率,在茶树种质资源的合理开发与利用中具有广阔的应用前景。运用优化后的RAPD反应体系对10个茶树品种的基因组DNA进行遗传差异分析,随机引物S89、S4分别在白毫早和福云6号中扩增得到长度为498bp、1 622 bp的差异片段,命名为BHZ498、FY1622。根据它们的测序结果分别设计了一对特异引物,BHZ498的特异引物为SB1/SB2;FY1622的特异引物为SC1/SC2,用这两对特异引物对10个茶树品种的基因组DNA进行扩增。引物SB1/SB2和SC1/SC2分别在白毫早和福云6号中扩增出唯一的一条扩增带,而这两对引物在其他供试茶树材料中均无相应的扩增带,结果表明已将BHZ498、FY1622标记成功转化成SCAR标记。

SCAR marker is one of new molecular markers based on RAPD technology. It could improve the efficiency of breeding assisted by molecular markers. It will show applied prospect in reasonable exploitation and utilization of the tea plant germplasm resources. The 10 tea cultivars were evaluated their genetic diversity by optimized RAPD reaction system. The specific fragments of 498 bp and 1 622 bp were amplified in Baihaozao and Fuyun-6 using random primer S89 and S4,named BHZ498 and FY1 622. According to their sequence of the PCR products, the specific primers were designed. SB1/SB2 was the specific primers of BHZA98 and SC1/SC2 was the specific primers of FY1622. The prospective results were obtained by amplification with these two pairs of specific primers in 10 tea cuhivars. The only one amplified band was acquired with SB1/SB2 in Baihaozao and attained with SC1/SC2 in Fuyun-6. These two pairs of primers had no corresponding amplified band in other tested materials. The results showed that RAPD marker had been transformed suecessfully into SCAR marker.