红平菇木质素降解酶系统漆酶、锰过氧化物酶及木质素过氧化物酶的检测

Detection on Laccase,Manganese Peroxidase and Lignin Peroxidase in Ligninolytic Enzymes of Pleurotus djamor

White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase(MnP),laccase and lignin peroxidase(LiP)activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470 nm,ABTS at 420 nm and veratryl alcohol(VA)at 310 nm,the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained.Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously,but no Lip.Substrates wood sawdust and 2,6-DMP could significantly enhanced MnP and laccase activities,the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1 when no substrates were added,and 69 U·L-1 and 205 U·L-1 when substrates added.The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.