摘要
目的通过对原发性肝细胞癌（ＨＣＣ）外周血中癌细胞的检测，为临床建立诊断肝癌转移的常规方法．．方法以甲胎蛋白（ＡＦＰ）ｍＲＮＡ为ＨＣＣ的标记物，应用ｎｅｓｔｅｄＲＴＰＣＲ对３０例ＨＣＣ外周静脉全血标本及不同细胞株进行检测及分析．结果ＨＣＣ患者３０例外周血癌细胞阳性检出率为５６７％，并与肝内静脉癌栓形成（１１／１３，８４６％ｖｓ６／１７，３５３％）、肝内转移（１６／２３，６９６％ｖｓ１／７，１４３％）和细胞分化密切相关（Ｐ＜００５）；最低检出浓度为全血肝癌细胞２×１０４／Ｌ．对照组中结肠癌、鼻咽癌等肝转移癌以及胆囊结石患者的外周血中均未发现ＡＦＰｍＲＮＡ．结论ＮｅｓｔｅｄＲＴＰＣＲ对ＨＣＣ的检测具有较强的特异性和较高的灵敏度，对于ＨＣＣ诊断。
AbstractAIM To develop a diagnostic method for metastasis in HCC through detecting the peripheral circulating hepatocellular carcinoma (HCC) cells .METHODS As a specific marker of HCC cells, AFP mRNA in circulating hepatocellular carcinoma cells was detected in 30 HCC patients and several other kinds of cell lines were examined by nested reverse transcript PCR (nested RTPCR).RESULTS AFP mRNA positivity in peripheral circulating HCC cells was 57% (17/30) and strongly related to portal vein tumor thrombus, intrahepatic metastasis and cell differentiation. With regard to controls, none of the samples showed AFP mRNA in peripheral circulating cells in colic and nasopharyngeal carcinoma with hepatic metastasis and cholecystolithiasis.CONCLUSION With high specificity and sensitivity, nested RTPCR for detecting peripheral circulating cells in HCC would be of great value in diagnosing metastasis of HCC.
