摘要
目的研究大肠癌患者血清及癌细胞内促胃液素（Ｇａｓ）水平及临床意义．方法采用ＲＩＡ法检测３５例大肠癌患者血清和癌细胞及癌旁粘膜细胞内Ｇａｓ水平．结果大肠癌患者术前、术后血清Ｇａｓ水平与对照组无显著差异（Ｐ＞００５），根治术后明显低于术前（２９ｎｇ／Ｌ±５ｎｇ／Ｌｖｓ３５ｎｇ／Ｌ±１２ｎｇ／Ｌ，ｔ＝２７７２，Ｐ＜００１），在高分化（３６ｎｇ／Ｌ±１６ｎｇ／Ｌｖｓ２８ｎｇ／Ｌ±５ｎｇ／Ｌ）和中分化腺癌组中（３８ｎｇ／Ｌ±７ｎｇ／Ｌｖｓ２７ｎｇ／Ｌ±３ｎｇ／Ｌ）差异显著（ｔ＝２１５２和２３５６，Ｐ＜００５）．大肠癌细胞内Ｇａｓ水平明显高于癌旁３ｃｍ和６ｃｍ粘膜（２１３ａｇ／细胞±７２ａｇ／细胞ｖｓ１４７ａｇ／细胞±３６ａｇ／细胞，１３９ａｇ／细胞±３２ａｇ／细胞；ｔ＝４８９１和５６１３，Ｐ＜００１）和正常粘膜（１３６ａｇ／细胞±４６ａｇ／细胞；ｔ＝２５３４，Ｐ＜００５），高分化腺癌明显高于低分化和粘液腺癌（２４１ａｇ／细胞±７８ａｇ／细胞ｖｓ１６１ａｇ／细胞±４６ａｇ／细胞，ｔ＝２５０５，Ｐ＜００５）．结论大肠癌细胞可通过自分泌方式分泌Ｇａｓ。
AbstractAIM To study the clinical significance of gastrin (Gas) levels in serum and cancer cells in patients with colorectal carcinoma.METHODS Gas contents in serum, cancer cells, canceradjacent mucosa cells (CAMC) were measured by radioimmunoassay in 35 patients with colorectal carcinoma.RESULTS Before and after operation, there was no significant correlation between the patients with colorectal carcinoma and the control (P>005) in the serum Gas levels. After radical operation the Gas levels in serum were obviously lower than before operation (29ng/L±5ng/L vs 35ng/L±12ng/L, t=2772, P<001), especially in highly and moderately differentiated adenocarcinoma (36ng/L±16ng/L vs 28ng/L±5ng/L, 38ng/L±7ng/L vs 27ng/L±3ng/L, t=2152 and 2356, P<005). The Gas levels in cancer cells were obviously higher than that in CAMC (213ag/cell±72ag/cell vs 147ag/cell±36ag/cell, 139ag/cell±32ag/cell; t=4891 and 5613, P<001) and normal mucosas cells (136ag/cell±46ag/cell, t=2534, P<005). The Gas levels in highly differentiated adenocarcinoma were obviously higher than that in poorly differentiated and mucinous adenocarcinoma (241ag/cell±78ag/cell vs 161ag/cell±46ag/cell, t=2505, P<005).CONCLUSION The colorectal carcinoma cells secrete Gas through autocrine. The elevation of Gas in colorectal carcinoma serves as a marker of well differentiation.
