芝麻种质资源叶绿素含量的多样性分析

Analysis of Diversity in Leaf Chlorophyll Content of Sesame Germplasm Resources

对116份芝麻种质资源在蕾期、盛花期和终花期的叶片叶绿素含量进行分析,结果表明:叶绿素含量从蕾期到终花期不断升高,种质间差异较大,改良品种(系)叶绿素含量高于地方种质;叶绿素含量的总体多样性指数为1.8950,地方种质的总体多样性指数(1.9094)高于改良品种(1.7740),地域来源不同的种质间多样性指数存在差异。利用欧氏最小距离法对3个生长时期的9项叶绿素含量相关指标进行聚类分析表明:聚类结果与种质来源和种质类型没有明显的规律性;供试种质明显被聚为具有62份种质(占53.45%)的低叶绿素含量和具有54份种质(占46.55%)的高叶绿素含量的两个大类群;综合筛选出山西八棱芝麻、驻芝11等17份高叶绿素含量的种质,可作为相关育种改良与遗传分析的研究材料加以利用。

Leaf chlorophyll contents of 116 sesame germplasm resources were analyzed at budding stage,full flowering stage and final flowering stage,respectively.The results showed that leaf chlorophyll content continually increased from budding stage to final flowering stage,there was biggish difference in leaf chlorophyll content among different germplasm resources,and the leaf chlorophyll contents in improved varieties were higher than those of local germplasm resources.The total diversity index of leaf chlorophyll content was 1. 8950, the difference in diversity index also existed among different area - derived germplasms, and the local germplasms had higher total diversity index （ 1. 9094） than that of the improved varieties （ 1. 7740）. Nine indexes correlative with leaf chlorophyll con- tent of 116 sesame germplasm resources at above three growth stages were analyzed by SPSS software according to Nearest Neighbor Eu- didean Distance, the results indicated that the clustering analysis results were not obviously related to the origin and types of germ- plasms, but the sesame germplasms used in this study could be divided into two types ： 62 germplasms （ 53.45% ） with low leaf chlorophyll content and 54 germplasms （46.55%） with high leaf chlorophyll content. Based on the above results, 17 germplasms with high leaf chlorophyll content such as ＂Shanxiballengzhima＂, ＂Zhuzhi No. 11＂ could be used as available research materials of genetic analysis and improvement breeding.