人胚食管异种移植和诱癌研究

A Study on Human Fetal Esophagus Heterotransplantation and Induced Carcinoma in Nude Mice

［目的］探讨人胚食管在裸鼠体内的异种移植及苯并在诱癌。［方法］人胚食管移植至探鼠，移植的人胚食管用苯并芘诱癌，观察移植物1－4个月并取材进行光镜、电镜和免疫级化检查。［结果］人胚食管在裸鼠皮下可存活4个月以上。移植的人胚食管用苯并芘诱癌，2个月可出现浸润性鳞状上皮癌。其病变有粘膜上皮坏死脱落、再生、增生、乳头状瘤样增生、不典型增生、原位癌及浸润癌。［结论］苯并芘能诱发人食管上皮癌。实验结果将有助于食管癌病因和发病机理研究。

[Purpose] To investigate the human fetal esophagus xenograft and Benzopyrene carcinogensis in nude mice[Methods] Human fetalesophagus segments were heterrotransplanted into nude mice Benzopyrene is used to induce carcinoma of the transplanted human embryo esophagus TheXenografts in nude mice were examined 1－4months after transplantation by light microscopy and electron microscopy and immunohistochemical meth－ods.[Results]Human embryo esophagus transplanted subcutaneously in the nude mouse could surrvive more than fourmonths Benzopyrene was used to induce epithelial carcinoma of the transplanted human embryo esophagus invasive carcinoma occurred two months later Pathologic change of epitheliummucosae consisted of necrosis exfoliation and hyperplasia; papilomatous hyperplasia; atypical hyperplasia; carcinoma in situ and invasive carcinoma[Conclusion]Human esophagealcarcinoma could be induced by Benzopyrene It provides a direct evidence that Benzopyrene is a causative agent of esophageal cancer The experiment results will help in the studies of the etiology and carcinogenic mechanism of esophageal carcinoma.