一株钾长石分解菌的分离·鉴定及系统发育

Isolation,Characterization and Phylogenetic Analysis of an Efficient Potassium Feldspar Decomposing Bacteria

[目的]分离、筛选具高效分解钾长石能力的菌株。[方法]从采自湖南省桂东县钾长石矿区的土壤中分离、筛选分解钾长石能力较强的菌株;通过形态观察、生理生化特征检测和基于16S rRNA基因序列的系统发育分析,对分离到的菌株进行初步鉴定。[结果]分离得到一株分解钾长石能力较好的JDM1菌株,初步鉴定为圆褐固氮菌的一个菌株;在温度为30℃、摇瓶转速为200 r/min的条件下培养96 h后,测得该菌发酵液中钾元素含量高达135.3 mg/L。[结论]菌株JDM1是一株具有较高分解钾长石性能的圆褐固氮菌。

[ Objective ] The research aimed to isolate and identify the efficient decomposing potassium feldspar bacterial strain. [ Method ] The efficient decomposing potassium feldspar bacterial strain was isolated from soil of potassium feldspar diggings of Guidong County of Hunan Province with the method of potassium feldspar dissolution selective cultivation. The isolated bacterial strain was identified by morphological, physiological and biochemical characters respectively, and 16S rRNA of the strain was sequenced. [ Result] The results of identification demonstrated that a potassium feldspar dissolution JDM1 strain was Azotobacter chroococcum. When the strain was cultured at 30 ℃ and 200 r/min for 96 h, the content of the soluble potassium was 135.3 mg/L. [ Conclusion ] JDM1 strain was an efficient potassium feldspar dissolution Azotobacter chroococcum.